| 幼畜繁殖（JIVET）技术在性成熟前奶牛上的应用 |
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| 引用本文： | 白佳桦,关宏,苟克勉,阎凤翔,崔秀宏,安晓荣. 幼畜繁殖（JIVET）技术在性成熟前奶牛上的应用[J]. 农业生物技术学报, 2010, 18(1): 187-191. DOI: 10.3969/j.issn.1674-7968.2010.01.028 |
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| 作者姓名： | 白佳桦 关宏 苟克勉 阎凤翔 崔秀宏 安晓荣 |
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| 作者单位： | 1. 北京市农林科学院畜牧兽医研究所,北京,100089
2. 中国农业大学农业生物技术国家重点实验室,北京,100193 |
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| 摘 要： | 本实验旨在建立高效的性成熟前奶牛幼畜繁殖(JIVET)技术体系.建立了有效的对性成熟前犊牛进行促性腺激素处理的方法,平均每只犊牛可获得卵母细胞31枚:卵母细胞在体外成熟和体外受精过程中,受精率达到63.2%,与成年牛(59.7%)差异不显著.胚胎体外培养后犊牛胚胎囊胚率达到31.6%,仍显著低于成年牛(48.0%).对3头同期发情受体母牛进行胚胎移植,其中2头受孕,1头完成全程妊娠,受孕率达到66.7%.
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| 关 键 词： | 犊牛 幼畜繁殖技术(JIVET) |
Application of Juvenile In vitro Embryo Transfer(JIVET) Technology on Prepubertal DairyCattle |
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| Bai Jiahua Guan Hong Gou Kemian Yan Fengxiang Cui Xiuhong An Xiaorong State Key Laboratories for Agrobiotechnology,China Agricultural University,Beijing,, Animal Husb,ry , Veterinary Research Institute,Beijing Academy of Agriculture , Foresty Scientific Research. Application of Juvenile In vitro Embryo Transfer(JIVET) Technology on Prepubertal DairyCattle[J]. Journal of Agricultural Biotechnology, 2010, 18(1): 187-191. DOI: 10.3969/j.issn.1674-7968.2010.01.028 |
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| Authors: | Bai Jiahua Guan Hong Gou Kemian Yan Fengxiang Cui Xiuhong An Xiaorong State Key Laboratories for Agrobiotechnology China Agricultural University Beijing Animal Husb ry Veterinary Research Institute Beijing Academy of Agriculture Foresty Scientific Research |
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| Affiliation: | Bai Jiahua 2* Guan Hong 1* Gou Kemian 1 Yan Fengxiang 1 Cui Xiuhong 1 An Xiaorong 1** 1 State Key Laboratories for Agrobiotechnology,China Agricultural University,Beijing,100193,2 Animal Husb,ry , Veterinary Research Institute,Beijing Academy of Agriculture , Foresty Scientific Research,100089 |
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| Abstract: | The objective of this experiment is to establish an effective juvenile in vitro embryo transfer(JIVET) technology system on prepubertal dairy cattle.In this experiment,an effective gonadotropic hormone stimulating protocol on calf was set up,and 31 oocytes per calf on average were obtained.After in vitro maturation and in vitro fertilization,the fertilization rate of calf oocytes reached 63.2%,which was no significant difference from matured bovine oocytes(59.7%).The blastocyst rate of calf embryos cultured... |
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| Keywords: | Calf Juvenile in vitro embryo transfer (JIVET) |
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