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杏鲍菇液体菌种的制备条件及应用研究
引用本文:孟丽君,王芳,张玉萍. 杏鲍菇液体菌种的制备条件及应用研究[J]. 山西农业大学学报(自然科学版), 2009, 29(2)
作者姓名:孟丽君  王芳  张玉萍
作者单位:山西省生物研究所,山西,太原,030006
摘    要:为了缩短杏鲍菇制种和生产周期,提高菇体产量,对杏鲍菇液体菌种的培养基和培养条件进行了筛选研究,结果表明其适宜的培养基组分为麸皮7%、葡萄糖2%、玉米粉1.5%、KH_2PO_40.1%、MgSO_40.05%、蛋白胨0.05%、VB_1 100 mg·L~(-1);适宜摇瓶的培养条件为:装量100 mL,培养温度26℃,摇床转速210 r·min~(-1),接种量15%。使用液体菌种做中间种能缩短原种和栽培种制种周期,也能缩短杏鲍菇栽培周期,并能提高杏鲍菇产量。

关 键 词:杏鲍菇  液体菌种  条件  应用

Study of Preparation Condition and Application of Liquid Spawn of Pleurotus Eryngii
MENG Li-jun,WANG Fang,ZHANG Yu-ping. Study of Preparation Condition and Application of Liquid Spawn of Pleurotus Eryngii[J]. Journal of Shanxi Agricultural University(Nature Science Edition), 2009, 29(2)
Authors:MENG Li-jun  WANG Fang  ZHANG Yu-ping
Abstract:Culture mediums and culturing conditions for liquid spawn of pleurotus eryngii were chosen and researched, the results indicated:the suitable culture medium group divided into wheat bran 7%,dextrose 2%,maize meal 1.5%, KH_2PO_4 0.1%,MgSO_4 0.05%,peptone 0.05%,VB_1 100 mg·L~(-1);the optimum filling level,culturing temperature,rotation speed and inoculum concentration were 100 mL,26℃,210 r·min~(-1)and 15%,respectively.Using liquid spawn not only shortens the whole cycle of spawn making and cultivation but also improves output of pleurotus eryngii.
Keywords:Pleurotus eryngii  Liquid spawn  Condition  Application
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