| 醋酸钙不动杆菌PAHs降解酶的超声波提取优化 |
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| 引用本文： | 张强,孙萍,王加宁,邱维忠,迟建国.醋酸钙不动杆菌PAHs降解酶的超声波提取优化[J].中国农学通报,2014,30(17):180-185. |
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| 作者姓名： | 张强 孙萍 王加宁 邱维忠 迟建国 |
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| 作者单位： | 1. 山东省科学院生物研究所2. |
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| 基金项目： | 国家高技术研究发展计划（863）;环保部公益性行业科研专项 |
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| 摘 要： | 为了提取醋酸钙不动杆菌 (Actnetobacter calcoaceticus)的PAHs降解酶，本文利用超声波破碎醋酸钙不动杆菌细胞，对多环芳烃（PAHs）降解酶进行优化提取。在对超声输出功率、工作\间隔时间、菌体稀释倍数以及总超声时间进行单因素实验的基础上，利用Box-Behnke中心组合实验设计，响应面法优化得到多环芳烃降解酶的最佳提取工艺为：工作\间隔时间为2s\2s、超声功率320W、总超声时间17 min、稀释12.5倍，在此条件下提取的酶活力为95.55U/g。
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| 关 键 词： | 贵定县 贵定县 |
| 收稿时间： | 9/2/2013 12:00:00 AM |
| 修稿时间： | 2013/10/21 0:00:00 |
Optimization of Ultrasonic disruption for PAHs-depredating enzymes from Actnetobacter calcoaceticus |
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| Abstract: | Ultrasonic wave was used in this paper to disrupt the cell of Actnetobacter calcoaceticus WG-6 for PAHs-depredating enzymes. The effect of ultrasound output power, duration of radiation each time, total irradiation time and the cell dilution multiple on the activity of PAHs depredating-enzymes were investigated through the single-factor experiment. The optimum conditions of PAHs depredating-enzymes extraction were determined through box-benhnken center-united design and response surface method were duration of radiation each time 2s (with a 2s interval), ultrasonic power 320W, total irradiation time 17 min, cell dilution multiple 12.5. The PAHs-depredating enzymes activity was 95.55U/g under this condition. |
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| Keywords: | response surface method |
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