转基因棉花抗虫基因的分子辅助选择

为提高棉花新品种选育过程中外源抗虫基因选择的准确性，以常规非转基因抗虫棉花品种、单价转基因抗虫棉品种、双价转基因抗虫棉品种为试验材料，选择能扩增Bt基因通用引物和棉花内源基因SAD1的引物，建立了抗虫基因的PCR检测方法。利用该方法对常规非转基因抗虫棉品系和转基因棉花品系进行了检测。结果表明，该方法可明确区分常规非转基因抗虫棉品系和转基因棉花品系；对高代育种品系进行检测表明40%转基因品系中存在单株不能扩增出预期的特异片段，而在非转基因系冈197中存在8.0%的单株扩增出预期的特异片段。该方法可应用于棉花新品种选育过程中对外源抗虫基因的检测，提高了选择的准确性和目的性。

Molecular Assisted Selection for Insect Resistant Gene (BT) in Transgenic Cotton

To improve the selection accuracy for exogenous insect resistant gene in cotton breeding, non-transgenic insect-resistant cotton varieties and the transgenic insect-resistant cotton varieties with single & two exogenous insect resistant genes were used to establish and optimize the PCR method for detection of exogenous insect resistant gene. Non-transgenic cotton lines and transgenic cotton lines were detected by Bt-specific primers and SAD1 primers (an endogenous gene in cotton), the results showed that the individual in 40% of the transgenic plant lines has no expected amplicon, 8.0% individual in the non-transgenic lines GANG 197 has expected amplicon. This method can be applied to cotton breeding for exogenous gene detection and improves selection accuracy.