长果皂荚皂苷提取工艺条件研究

对湖南都庞岭自然保护区特有植物——长果皂荚的皂苷提取工艺条件进行研究，采用水浸提方法，考查了提取温度、时间、次数、固液比对长果皂荚总皂苷提取效率的影响。结果表明，经过一系列的单因素实验和L16(43)正交实验，得到了长果皂荚皂苷提取工艺的最佳条件：在浸提温度70℃、时间8 h、首次浸提固液比为1:4，第2-n次为1:2，浸提4次的条件下，可获得最高总皂苷提取率29.67%，为进一步开发利用长果皂荚提供实验依据。

Research on Extraction Process Conditions of Sapoin from Legume of Gleditsia longoleguminosa

The extraction technology of sapoin from legume of Gleditsia longoleguminosa was established, which was an endemic plant in the Dupangling Natural Reserve. The results showed that: by a serials of single-factor experiments and L16(43) orthographic experiments, the extraction conditions were optimized: the legume powder of Gleditsia longoleguminosa was leached by water with 4 folds of its weight at the first time, the subsequent was kept on 2 folds, the leaching time was 8.0 h, the temperature was 70℃, and the leaching frequency was 4 times in total. Under these conditions, the total sapoin extraction yield could reach up to 29.67%. This research offered an important experimental reference for further developments and utilizations of Gleditsia longoleguminosa.