7个柱花草品种的AFLP和SSR遗传多样性分析

旨在阐明不同柱花草品种的遗传差异。利用AFLP、SSR两种分子标记技术对7个柱花草品种进行遗传多样性分析。12对AFLP引物组合共扩出966个条带，其中多态性条带有850条，平均每对引物的多态性条带为70.8条，多态性比率为87.99%。10对SSR引物共扩出26个条带，其中多态性条带有23个，多态性比率为88.46%；10对引物的观察杂合度(Ho)的范围是0~0.7143，平均为0.1857，Shannon’s遗传多样性指数(I)平均值为0.6545，平均多态性含量(PIC)为0.727。根据非加权成对平均数法(UPGMA)进行聚类分析，获得品种聚类树形图，AFLP与SSR分析的聚类图相似性显著，7个品种明显分成两类。‘矮柱花草’与‘西卡柱花草’的遗传关系最为疏远，‘奥克雷柱花草’与‘爱德华柱花草’、‘格拉姆柱花草’与‘热研5号柱花草’关系最为密切。

The Assessment of Genetic Diversity of Severn Stylosanthes guianensises by Using AFLP and SSR Analysis

In order to study the genetic diversity of different varieties of Stylosanthes guianensises, seven Stylosanthes guianensises were analyzed by AFLP and SSR markers. About 88 percent of 966 bands produced by using twelve AFLP primer combinations were polymorphic. The average polymorphic bands of each pair of primers were 70.8. 10 pairs of SSR primers generated 26 bands in which 21 bands were polymorphic. The range of the observed heterozygosity in 10 pairs of SSR primers was between 0 and 0.7143 with an average of 0.1857. The average of Shannon’s information index and the average content of polymorphism were 0.6545 and 0.727. Clustering tree was ploted based on UPGMA. The analysis results of AFLP and SSR markers were exactly similar. Seven Stylosanthes guianensises were divided into two groups. The genetic relationship between Stylosanthes humilis HBK and Stylosanthes scabra cv. Seca was the farthest and the genetic relationships between Stylosanthes guianensis cv. Oxley and Stylosanthes guianensis cv. Endeavour, Stylosanthes guianensis cv. Graham and Stylosanthes guianensis cv. Reyan No.5 were the closest.