| 猪羊生长激素基因保守性研究 |
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| 引用本文： | 郑新民,华文君,乔宪凤,赵浩斌,李莉,曹军平,周荆荣,魏庆信. 猪羊生长激素基因保守性研究[J]. 湖北农业科学, 2001, 0(2): 62-64 |
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| 作者姓名： | 郑新民 华文君 乔宪凤 赵浩斌 李莉 曹军平 周荆荣 魏庆信 |
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| 作者单位： | 1. 湖北省农业科学院生物技术研究所
2. 湖北省农业科学院生物技术研究所武汉 430064 |
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| 基金项目： | 国家863资助项目(编号：101-05-01-01) |
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| 摘 要： | 生长激素基因在猪羊间的差异 ,通过两对引物 ,分别为P1:5’ -agctggattctttacggctgagcc - 3’和P2 :5’ -tccggaagcaggagagcagaccgtag - 3’ ,P3:5’ -gctgacacctacaaggagtttg - 3’和P4:5’ - gtttgcttgaggatctgtcctg - 3’。对它们的DNA进行聚合酶链反应 ,电泳及序列分析结果表明 :P1-P2和P3-P4引物都能扩增出猪羊DNA样 ,得到大小一致的扩增带 (对应引物的带 ) ,但southern杂交结果的差别很大 ;生长激素基因的碱基组成在猪羊间的差异为 6 5 % ,即猪羊生长激素基因保守性很强
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| 关 键 词： | 猪 羊 生长激素基因 保守性 |
| 修稿时间： | 2000-10-12 |
Study on the conservation of swine and goat growth hormone gene |
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| Zheng Xinmin. Study on the conservation of swine and goat growth hormone gene[J]. Hubei Agricultural Sciences, 2001, 0(2): 62-64 |
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| Authors: | Zheng Xinmin |
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| Abstract: | this experiment is to study growth hormone gene difference between swine and goat by PCR with two pairs primers including P1:5' agctggattctttacggctgagcc 3'and P2:5' tccggaagcaggagagcagaccgtag 3',P3:5' gctgacacctacaaggagtttg 3'and P4:5' gtttgcttgaggatctgtcctg 3'.Their templets were from swine DNA and goat DNA.electrophoresis,blot and sequences analysis results shown P1 P2 and P3 P4 primers can produce similar DNA bands.but southern blot result was big difference.Growth hormone gene difference is 6 5% between swine and goat.This means growth hormone gene to have good conservation between swine and goat. |
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| Keywords: | swine goat growth hormone gene conservation |
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