Simultaneous identification of species and trichothecene chemotypes of <Emphasis Type="Italic">Fusarium asiaticum</Emphasis> and <Emphasis Type="Italic">F. graminearum</Emphasis> sensu stricto by multiplex PCR |
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Authors: | Fumihiko Suzuki Ayako Koba Takashi Nakajima |
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Institution: | (1) Research Team for Fusarium Head Blight Control, National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi Kumamoto, 861-1192, Japan |
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Abstract: | A multiplex PCR assay was developed for simultaneous identification of the species and trichothecene chemotypes for Fusarium asiaticum and F. graminearum sensu stricto based on the genes related to trichothecene biosynthesis. PCR was carried out in a single reaction with three
pairs of primers designed for the tri6 region and one pair of primers designed for tri3. We confirmed that the multiplex PCR was able to identify species and chemotypes for all tested strains of F. asiaticum and F. graminearum s. str. isolated in Japan. This technique would be a useful and rapid tool for diagnosis, epidemiology, and population structure
studies of the F. graminearum complex in Japan. |
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