| 真空渗入法转pinⅡ基因菜薹外源基因的遗传与表达 |
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| 引用本文： | 徐恒戬,刘凡,王秀峰,赵泓,曹传增,罗晨.真空渗入法转pinⅡ基因菜薹外源基因的遗传与表达[J].园艺学报,2004,31(4):511-513. |
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| 作者姓名： | 徐恒戬 刘凡 王秀峰 赵泓 曹传增 罗晨 |
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| 作者单位： | ( 山东农业大学园艺学院，泰安271018； 国家蔬菜工程技术研究中心，北京100O89； 北京市农林科学院植保环保所，北京100089) |
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| 基金项目： | 北京市高技术实验室项目资助(953850100) |
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| 摘 要： | 本研究对利用真空渗入法获得的转bar基因及pinⅡ基因菜薹，进行了外源基因的遗传及表达情况分析。结果表明通过该方法获得的转基因植株，外源基因能够稳定遗传。bar基因在 代的分离大部分符合孟德尔遗传规律，但也有不符合的群体；pin II基因在 代中稳定表达，但各个株系之间的表达水平差异较大，因而各个株系的抗虫效果也具有显著差异，即使具有相同外源基因拷贝数的各个株系，其PIN I／蛋白表达量和抗虫效果也具有显著差异。
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| 关 键 词： | 菜薹 pinII基因 抗虫 基因表达 |
| 文章编号： | 0513-353X(2004)04-0511-03 |
Heredity and Expression of Foreign Genes in pinⅡ Transgenic Flowering Chinese Cabbage Obtained by Vacuum Infiltration |
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| Xu Hengjian,Liu Fan,Wang Xiufeng,Zhao Hong,Cao Chuanzeng,and Luo Chen.Heredity and Expression of Foreign Genes in pinⅡ Transgenic Flowering Chinese Cabbage Obtained by Vacuum Infiltration[J].Acta Horticulturae Sinica,2004,31(4):511-513. |
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| Authors: | Xu Hengjian Liu Fan Wang Xiufeng Zhao Hong Cao Chuanzeng and Luo Chen |
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| Institution: | ( Collage ofHorticulture，Shandong Agricultural University，T~i'an 271018，China； National Engineering Research CenterforVegetables，Beijing 100089，China； Institute of Plant and Environmental Protection，Beijing Academy ofAgriculture and Forest—ry，Beijing 100089，China) |
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| Abstract: | Transgenic Chinese cabbage (Brassica campestris L．ssp．chinensis var．utilis Tsen et Lee)with pin n gene and bar gene were got by vacuum infiltration transformation and strictly self-crossed．PCR assay，Basta resistance and insect resistance of T2 generation lines proved stable inheritance and expression of theforeign genes．The segregation ratios of 41 lines indicated low—copy transformation，and majority of them accord—ing with the Mendel’S segregation law．The resistance of transgenic lines to Plutella xylostella L．has significantdiference along with the variation of relative quantities of PIN II protein．even lines with the san3．e insertion copies． |
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| Keywords: | Flowering Chinese cabbage pin n gene Insect resistance Gene expression |
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