东北林蛙体表两种常驻细菌的分离鉴定

以自然状态下健康东北林蛙(Rana dybowskii)体表常驻菌群为研究对象,用无菌水漂洗掉东北林蛙体表暂住菌后,用无菌拭子收集蛙体表常驻菌并进行培养,之后对单菌落进行分离纯化。通过对菌落形态观察、生化反应管等生化方法测定及16S rDNA序列检测,两方法同时鉴定出东北林蛙体表常驻细菌2种:醋酸钙不动杆菌(Acinetobacter calcoaceticus)和产碱假单胞菌(Pseudomonas alcaligenes),属于环境常见革兰氏阴性细菌。根据结果推测东北林蛙体表常驻细菌来源于环境,并对其生物体起到一定的保护作用。

Isolation and Identification of Bacteria on the Surface of Rana dybowskii

The objective of this study was to characterize the resident bacterial flora on the skin of healthy Rana dybowskii living in natural field state.After rinsed transient bacteria from the skin with sterile water,resident bacteria from frog skin were sampled using sterile swabs and cultured,and single colony was isolated and purified.After observation of colony morphology,biochemical reactions and sequencing of the 16S rDNA gene were used to identify the bacteria.Then two bacterial species from Rana dybowskii skin were identified to be related to the species Acinetobacter calcoaceticus and Pseudomonas alcaligenesby biochemistry and molecular methods.Both the bacteria were found gram negative and common in the environment.This results suggested that Rana dybowskii skin resident bacteria came from the environment and might have a protective role in preventing infection.