中华鳖日本品系和清溪乌鳖线粒体12S rRNA基因部分序列分析

采用PCR产物直接测序法测定了中华鳖日本品系和清溪乌鳖2个国家水产新品种线粒体12S rRNA基因部分序列,克隆获得了12S rRNA基因992个可比对位点。中华鳖日本品系和清溪乌鳖核苷酸A、T、C、G含量相似,平均含量分别为21.90%、37.20%、16.53%、24.37%;22.00%、37.54%、16.35%、24.12%。除1处插入/缺失位点外,两者序列间有11个变异位点,其中转换1个、颠换10个,得到了3个12S rRNA基因单倍型。使用NJ法对包括GenBank报道的安徽和韩国中华鳖在内的5种12S rRNA单倍型进行聚类分析,结果表明:中华鳖日本品系与韩国产中华鳖聚为一支,清溪乌鳖与安徽产中华鳖聚为一支。

Sequence Composition of Mitochondrial 12S rRNA Genes between Two Varieties of Pelodiscus sinensis

Partial sequences of the mitchondrial 12S rRNA gene of two Pelodiscus sinensis varieties were determined.992 comparable base pairs were obtained,which were with similar A,T,C and G contents,21.90%,37.20%,16.53%,24.37% and 22.00%,37.54%,16.35%,24.12% for Japanses and Wubie variety respectively.Except one deletion/insertion site,there were 11 different sites(1 transition site and 10 transversion sites) between the two varieties,forming 3 haplotypes.Topological structure of the molecular phylogenetic tree was constructed by homologous segments of 12S rRNA gene from 5 haplotypes(3 in this study,1 Korea and 1 Anhui from Genbank) with Neighbor-Joining method.The results showed that Jap haplotypes and Korea haplotype were clustered into distinct clades,while Wu haplotype and Anhui haplotype were clustered together.