猪圆环病毒2型对体外培养的仔猪淋巴细胞抗氧化能力的影响

为进一步了解猪圆环病毒2型(PCV-2)引起淋巴细胞凋亡的机理,测定了PCV-2对体外培养的仔猪淋巴细胞抗氧化能力的影响。取培养后0,2,6,24,48 h的上清液和细胞,用试剂盒测定上清液中总抗氧化能力、一氧化氮(NO)含量、超氧阴离子自由基活性和超氧化物岐化酶活力的变化,同时测定淋巴细胞内一氧化氮合酶(NOS)活力。结果表明,试验组与对照组总抗氧化能力无显著性差异(P>0.05);试验组抗超氧阴离子自由基活性与对照组相比,除了0 h,其他时间点试验组均显著高于对照组(P<0.05);总超氧化物岐化酶活力试验组和对照组均维持在19 U/mL左右,无显著差异(P>0.05);NO含量除24 h时试验组高于对照组外(P<0.05),其余时间两组间差异不显著;细胞内NOS活力除0 h外,其余时间均是对照组高于试验组,且2、24 h显著增高(P<0.05);对照组诱导型一氧化氮合酶(iNOS)活力6 h时显著高于试验组(P<0.05),其余时间(0 h除外)也较试验组高。

In vitro antioxide capacity of piglets lymphocytes infected by porcine circovirus 2

In order to further find out the mechanism of lymphocytes apoptosis induced by porcine circovirus 2(PCV-2),we measured the in vitro antioxide capacity of piglets splenic lymphocytes infected by PCV-2.The supernatant liquid and cells at 0,2,6,24 and 48 h after culture were used to investigate the total antioxidant capacity,content of nitric oxide(NO) and activities of superoxide anion free radical,superoxide dismutase,nitric oxide synthase(NOS) and induced nitric oxide synthase(iNOS).The results indicated there was no significant difference in the total antioxidant capacity between treatment group and control(P>0.05);the superoxide anion free radical activity of treatment group was significantly higher than that of the control(P<0.05)except at 0 h;there was no significant difference in the antisuperoxide anion free radical capability between treatment group and control(P>0.05),and both kept a same level of 19 U/mL;There was no significant difference between the two groups except that at 24 h,the content of NO in treatment group was higher than that of the control(P<0.05);The activity of NOS in treatment group was lower than that in the control,especially at 2 h and 24 h(P<0.05);The activity of iNOS in treatment group was lower than that in the control except at 0 h,especially at 6 h,the difference was significant(P<0.05).