| ELISA法检测猪瘟抗体消长规律的试验研究 |
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| 引用本文： | 李国江,胡娜,张立春. ELISA法检测猪瘟抗体消长规律的试验研究[J]. 吉林农业科技学院学报, 2009, 18(2): 21-23 |
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| 作者姓名： | 李国江 胡娜 张立春 |
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| 作者单位： | [1]吉林农业科技学院动物医学学院,吉林132101 [2]吉林农业科技学院05级动物医学专业学生,吉林132101 |
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| 摘 要： | 本试验采用间接ELISA方法,对猪瘟（CSF）常规免疫的仔猪CSF抗体水平进行检测。试验分A、B两组,A组首免注射4头份猪瘟兔化弱毒疫苗,B组首免注射2头份猪瘟兔化弱毒疫苗;二免两组均注射2头份猪瘟兔化弱毒疫苗。试验结果表明,注射疫苗后10d,开始产生抗体,20~30d抗体达到峰值,二免后抗体峰值维持40d以上。首免4头份的仔猪比首免2头份的仔猪CSF抗体峰值出现的早,维持的时间长。
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| 关 键 词： | 猪瘟 抗体 ELISA 检测 |
A Study on the Test of the Regular of ELISA Mthod to Dtect Classical Swine Fever Antibody |
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| LI Guojiang,HU Na,ZHANG Lichun. A Study on the Test of the Regular of ELISA Mthod to Dtect Classical Swine Fever Antibody[J]. Journal Of Jilin Agricultural Science And Technology College, 2009, 18(2): 21-23 |
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| Authors: | LI Guojiang HU Na ZHANG Lichun |
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| Affiliation: | 1. Jilin Agicultural Science and Technology College College of Animal Medicine, Jilin 132101, China ; 2. Jilin Agicultural Science and Technology College A student in Grade 05 Class Animal Medicine, Jilin 132101, China) |
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| Abstract: | Indirect ELISA method is applied to detect classical swine fever antibody of rutine immunization in piglets. The experiment is divided into A and B groups, A group of piglets are used 4 copies of classical swine fever in rabbits attenuated in the first immunization, B group of piglets are used 2 copies of Classical swine fever in rabbits attenuated in the first immunization;Two groups piglets are used 2 copies of classical swine fver in rabbits attenuated in the second immunization. The experiment results show that antibody produce in the tenth day after vaccine injected, antibody peak appear in the twentieth to thirtieth day after vaccine injected, antibody peak is maintained more than forty days after the second immunization. In the first immunization 4 copies piglets antibody peak appear earlier than 2 copies piglets, moreover maintain time longer. |
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| Keywords: | classical swine fever antibody ELISA detect |
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