Influence of regeneration and selection procedures on the production of aluminium tolerant potato regenerants

Summary Tests were made of the influence of selective and nonselective culture media on the rate of production of aluminium tolerant regenerant clones. Regenerants died when selective medium containg Al was continuously applied in tolerance tests. The production of aluminium tolerant clones was dependent on alternating selective and nonselective media. Tests using a medium containing 6 mmol al resulted in 50% growth as compared to control plants. Regenerants showed no growth when a medium with 10 mmol Al was applied. No constant tolerance was obtained when only a nonselective medium was used for regneration.