| 2种改进的未知细菌DNA提取方法 |
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| 引用本文： | 王庆容,李顺,宋培勇,杨秀荣.2种改进的未知细菌DNA提取方法[J].安徽农业科学,2009,37(12):5381-5382. |
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| 作者姓名： | 王庆容 李顺 宋培勇 杨秀荣 |
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| 作者单位： | 遵义师范学院生物系,贵州遵义,563002;遵义师范学院生物系,贵州遵义,563002;遵义师范学院生物系,贵州遵义,563002;遵义师范学院生物系,贵州遵义,563002 |
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| 基金项目： | 贵州省遵义市科技局资助项目(遵市科合社字2007-21号) |
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| 摘 要： | 目的]研究细菌DNA的提取方法，为未知细菌的研究提供方法支持。方法]从DNA的纯度、基因组DNA的酶切效果、16SrRNA基因扩增等方面比较了细菌DNA的2种提取方法。结果]方法Ⅰ提取的DNA的纯度（OD260／OD280）为1．51-1．65，6个样品的平均值为1．56。方法Ⅱ提取的DNA的纯度（0D260／OD280）为1．25～1．41，6个样品的平均值为1．33。方法隈取的DNA纯度较高，但其试验步骤稍微繁琐。方法Ⅱ的试验步骤稍微简单，但试验时间较长。2种方法均提取到长度大于21kb）的细菌基因组DNA。对2种方法提取的DNA进行PCR扩增都得到长度约600bp的条带，效果较好。结论]2种方法提取的DNA质量均较高，都可以直接用于RFLP、PCR等研究。
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| 关 键 词： | 细菌 DNA提取 聚合酶链反应 |
Two Improved Extraction Methods of DNA in Unknown Bacteria |
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| WANG Qing-rong et al.Two Improved Extraction Methods of DNA in Unknown Bacteria[J].Journal of Anhui Agricultural Sciences,2009,37(12):5381-5382. |
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| Authors: | WANG Qing-rong |
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| Institution: | WANG Qing-rong et al(Biological Department,Zunyi Normal College,Zunyi,Guizhou 563002) |
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| Abstract: | Objective] The aim was to study the extraction method of DNA in bacteria so as to supply methodological support for studying unknown bacteria.Method] The 2 extraction methods of DNA in bacteria were compared in aspects such as purity of DNA,enzyme digestion effect of genome DNA and gene amplification of 16S rRNA.Result] The purity of DNA(OD260/OD280)extracted by method 1 was between 1.51 and 1.65 and the average purity of the 6 samples was 1.56.The purity of DNA extracted by method 2 was between 1.25 and... |
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| Keywords: | Bacteria DNA extraction Polymerase chain reaction |
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