NaCl胁迫对菜用大豆种子膨大初期蛋白质表达的影响

采用蛭石栽培，对开花后10 d的菜用大豆 [Glycine max (L.) Merr.] 进行100 mmol/LNaCl处理，利用双向电泳（Two-dimensional gel electrophoresis, 2-DE）技术对胁迫5 d时的种子蛋白质进行分离，并对处理与对照2-DE图谱中蛋白质表达进行比较分析。在对照和处理的2-DE图谱上均检测到327个蛋白点，有28个差异表达蛋白，其中16个较对照显著上调，另外12个显著下调。利用基质辅助激光解吸离子化飞行时间质谱（Matrix-assisted laser desorption ionization time-of-flight mass spectrometry，MALDI-TOF-MS）对其中6个丰度差异较大（丰度变化在2.5倍以上）的蛋白进行分析，通过数据库检索，从中鉴定出5个蛋白质，分别是大豆球蛋白前体G2、肌动蛋白、大豆球蛋白前体G2相似蛋白、类-微管蛋白和Kunitz型胰蛋白酶抑制剂，并对这些蛋白质在NaCl胁迫下可能的作用进行了讨论。结果表明，NaCl胁迫对菜用大豆种子膨大初期的蛋白质代谢产生了显著影响，肌动蛋白、类-微管蛋白和Kunitz型胰蛋白酶抑制剂可能参与了对NaCl胁迫的应答反应。

Effect of NaCl stress on protein expression of vegetable soybean seed at early filling stage

Under vermiculite culture conditions,vegetable soybean [Glycine max(L.) Merr.] was treated with 100 mmol/L NaCl at the 10th day after flowering.After the separation of proteins from the seeds at the 5th day of the NaCl stress by two-dimensional gel electrophoresis(2-DE),the differences in protein expression were analyzed between the NaCl treatment and the control.About 327 proteins are detected at each 2-DE map,of which 28 proteins are found to be differentially expressed.Among these NaCl-stress-changed proteins,16 proteins are up-regulated,while other 12 proteins are down-regulated significantly.Out of the 28 proteins,the abundances{JP2 of 6 proteins are changed more than 2.5 times.The 6 proteins are analyzed by using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and searched in the database which allows the identification of 5 spots.These identified proteins are Glycinin G2 precursor,actin,similar to GLYCININ G2 PRECURSOR,alpha-tubulin-like protein and Kunitz trypsin inhibitor.The protein metabolisms at early seed-filling of vegetable soybean of the identified proteins are influenced significantly under the NaCl stress,and actin,alpha-tubulin-like protein and Kunitz trypsin inhibitor might be involved in NaCl stress responses.