高效氯氰菊酯对草地贪夜蛾Sf9细胞自噬的诱导作用

采用噻唑蓝（MTT）染色、显微镜及透射电子显微镜观察、单丹磺酰戊二胺（MDC）荧光染色及蛋白质免疫印迹（WB）技术等方法，分别针对高效氯氰菊酯（beta-cypermethrin）作用后草地贪夜蛾Spodoptera frugiperda卵巢细胞系Sf9细胞的细胞活力、自噬发生的典型特征及自噬相关蛋白变化情况进行了测试及分析。结果表明:经不同浓度高效氯氰菊酯处理后，对草地贪夜蛾Sf9细胞活性的抑制效应与作用剂量及时间呈现依赖关系，并表现出典型的细胞自噬现象；LC3-Ⅱ/LC3-Ⅰ蛋白表达水平比和Beclin 1蛋白表达量呈现上调趋势，分别为对照组的201.32%和136.00%，p62蛋白表达量下调至对照组的68.13%。研究表明，高效氯氰菊酯对草地贪夜蛾Sf9细胞具有显著的细胞毒性，并且其对细胞活力的抑制作用与自噬诱导途径相关。

Beta-cypermethrin induced Spodoptera frugiperda Sf9 cells autophagy research

The cell viability and autophagic vacuoles of the beta-cypermethrin treated Sf9 cells (Spodoptera frugiperda cell) were evaluated and the autophagic-related proteins of Sf9 cells was analyzed. 3-(4,5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide(MTT) assay, microscope assay, transmission electron microscope(TEM) assay, monodansylcadaverine(MDC) fluorescent staining assay and western blot(WB) assay were employed for the investigation. Results showed that beta-cypermethrin inhibited the viability of Sf9 cells in both concentration- and time-dependent manners, and the autophagy was observed in beta-cypermethrin-treated cells. Autophagy-related protein LC3-Ⅱ/LC3-Ⅰand Beclin 1 were up-regulated to 201.32% and 136.00% compared to the control group. And protein p62 was down-regulated to 68.13%. It has been demonstrated that beta-cypermethrin has significant cytotoxicity on Sf9 cells and inhibition the cell's ability via the autophagy pathway.