| 7种菊科药用植物的RAPD分析 |
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| 引用本文： | 樊晓霞,颜松,唐静仪,茆灿泉,李迪强,王万军.7种菊科药用植物的RAPD分析[J].安徽农业科学,2010,38(17):8964-8966. |
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| 作者姓名： | 樊晓霞 颜松 唐静仪 茆灿泉 李迪强 王万军 |
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| 作者单位： | 西南交通大学生命科学与工程学院,四川成都,610031;中国林业科学院森林生态环境与保护研究所,北京,100091 |
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| 基金项目： | 国家科技部自然科技资源平台子项目 |
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| 摘 要： | 目的]利用RAPD技术研究7种菊科药用植物的遗传多样性及亲缘关系。方法]从50条10 bp随机引物中筛选出10个多态性好的引物进行RAPD扩增,扩增DNA片段数据用UPGMA聚类法构建系统发育树。结果]10条引物共扩增出337条带,多态性带337条。聚类结果显示,RAPD分子标记构建的系统发育树在族内属间与传统分类系统一致。结论]该研究可为菊科药用植物的鉴别提供参考,并为针对性地进行菊科植物的保护提供依据。
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| 关 键 词： | 菊科 药用植物 RAPD分析 |
RAPD Analysis of Seven Species of Medical Plants from Asteraceae |
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| FAN Xiao-xia et al.RAPD Analysis of Seven Species of Medical Plants from Asteraceae[J].Journal of Anhui Agricultural Sciences,2010,38(17):8964-8966. |
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| Authors: | FAN Xiao-xia |
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| Institution: | FAN Xiao-xia et al(College of Life Sciences and Engineering,Southwest Jiaotong University,Chendu,Sichuan 610031) |
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| Abstract: | Objective]The research aimed to analyze 7 species of medical plants from Asteraceae by random amplified polymorphic DNA(RAPD) analysis.Method] 7 species of medical plants from Asteraceae were amplified by 10 selected primers from 50 primers,and the results were generated by UPGMA algorithm to cluster genetic distance caculated from RAPD data.Result] 10 selected primers were able to produce 337 clear polymorphic bands which were all polymorphic.The genetic results based on RAPD markers were consistant wit... |
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| Keywords: | Asteraceae Medical plants RAPD analysis |
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