褐藻胶裂解酶产生菌的发酵优化研究

以筛选的羊栖菜腐败菌系不动杆菌属X8菌株为研究对象,对其产褐藻胶裂解酶的发酵培养条件进行优化研究.结果表明,该菌株最适液体培养基组成为(m/V):0.6%褐藻酸钠,0.7%蛋白胨,0.2% KH_2PO_4,0.2 % NaCl,初始pH 7.0.该菌株于25 ℃,150 r/min 振荡培养16 h后,褐藻胶裂解酶酶活可达157.32 U/ml,较优化前提高了1.59倍.Mg~(2+)、Ca~(2+)和Fe~(2+)对菌株产酶有一定的抑制作用.

Optimization of Alginate-degrading Bacterial Fermentation Process for Alginate Lyase Production

The enzyme production of a bacterium strain with relatively high alginate lyase activity known as Acinetobacter X8 isolated from decaying part of seeweed Sargassum fusiform was investigated under liquid fermentation. The optimized liquid fermentation medium(m/V) was comprised of 0.6 % alginate, 0.7 % peptone, 0.2 % K_2HPO_4, and 2 % NaCl at initial pH of 7.0. After fermentation for 16 h at 150 r/min and 25 ℃, the yield of alginate lyase was found to be up to 157.32 U/ml, 159.01% higher than that under the ordinary conditions. However, the production process was inhibited by Mg~2, Ca~(2+) and Fe~(2+).