| F小体检测奶牛XY精子纯度 |
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| 引用本文： | 董琴,孟浩,马文强,高庆华.F小体检测奶牛XY精子纯度[J].塔里木农垦大学学报,2013(3):43-47. |
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| 作者姓名： | 董琴 孟浩 马文强 高庆华 |
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| 作者单位： | [1]新疆塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 |
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| 基金项目： | 国家自然科学基金资助项目（31060306,30760170） |
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| 摘 要： | 为了建立F小体检测奶牛精子纯度的方法，试验采用正交设计检测精子F小体不同的胰酶消化时间（40min，60min，80min）、喹吖因染色浓度（0．4％，0．5％，0．6％）和染色时间（10min，20min，30min）的优化方案，并且对分选X、Y精子及常规精液的F小体进行染色检测，评价分选X、Y精子的纯度。结果显示分选x、Y精子及常规精液在0．25％胰酶消化20min后0．5％喹吖因染色10min的条件下，纯度分别为92．7％、92．5％、48．3％与对应精子的理论纯度（93．3％，93．7％，50％）差异均不显著（P〉0．05）。因此本实验建立的F小体检测法可用于XY精子纯度的检测。
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| 关 键 词： | F小体 精子比率 纯度评价 |
The Purity Assessment of X- and Y- sperm from Dairy Cow Using F body |
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| Dong Qin Meng Hao Ma Wenqiang Gao Qinghua.The Purity Assessment of X- and Y- sperm from Dairy Cow Using F body[J].Journal of Tarim University of Agricultural Reclamation,2013(3):43-47. |
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| Authors: | Dong Qin Meng Hao Ma Wenqiang Gao Qinghua |
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| Institution: | Dong Qin Meng Hao Ma Wenqiang Gao Qinghua. (1 College of Animal Science, Tarim University, Alar, Xinjiang 843300;2 Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production and Construction Groups Tarim University, Alar, Xinjiang 843300) |
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| Abstract: | To establish the F body detection method for purity assessment of sperm, this experiment optimized the digestion time （40, 60, 80 min）, quinacrine concentration （0. 4, 0. 5, 0. 6% ） and dyeing time（ 10, 20, 30 min） of the F body detection with orthogonal design. By using optimum method the sperm sex ratio of sex - sorted and unsorted sperm were investigated though F body detection. The results showed that the optimal conditions was defined as 0. 25% trypsin digestion for 20 min and staining 10 min. Under the above condition The parity were 92. 7%, 92. 5% and 48. 3% ,the Theoretica/puritywere 93. 3% ,93. 7% ,50% , there was no significant difference （ P 〉 0. 05 ）. So the Fbody detection method can be used to detect the puruty of X - and Y - sperm. |
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| Keywords: | F- body sperm sex ratio purity assessment |
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