| 番茄BADH2基因转化水稻 |
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| 引用本文： | 刘俊利. 番茄BADH2基因转化水稻[J]. 安徽农业科学, 2006, 34(23): 6138-6139,6184 |
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| 作者姓名： | 刘俊利 |
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| 作者单位： | 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川成都,610064 |
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| 基金项目： | 四川大学生命科学学院资助项目 |
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| 摘 要： | 用分子生物学手段,构建含番茄BADH2基因的过表达载体,通过农杆菌介导的方法将其导入日本晴幼胚诱导的愈伤组织,获得了303块潮霉素(Hm)抗性愈伤组织。获得1 059个转化植株。随机选择115个T0植株进行Hyp离体叶片筛选,结果表明,绝大多数植株均呈抗性,并且与PCR检测结果相吻合,说明T0植株中不存在假阳性。
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| 关 键 词： | 番茄 水稻 农杆菌 基因转化 BADH2基因 |
| 文章编号： | 0517-6611（2006）23-6138-02 |
| 收稿时间： | 2006-08-22 |
| 修稿时间： | 2006-08-22 |
Transgenic Rice with a Gene Encoding for the Betaine Aldehyde Dehyrogenase 2(BADH2)of Tomato |
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| LIU Jun-li. Transgenic Rice with a Gene Encoding for the Betaine Aldehyde Dehyrogenase 2(BADH2)of Tomato[J]. Journal of Anhui Agricultural Sciences, 2006, 34(23): 6138-6139,6184 |
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| Authors: | LIU Jun-li |
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| Affiliation: | College of Life Sciences, Siehuan Univensily; Key laboratory of Bio-resoarees and Eco-environment Ministry of Education, Chengdu, Sichuan 610064 |
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| Abstract: | With the molecular bio-technology,the calli derived from young embryos of japonica rice cultivars were transferred with BADH2 gene of tomato,which had been constructed to be overexpression vector mediated with Agrobacterium.A total of 303 independent Hygromycin(Hm) resistant calli were obtained,which produced 1059 transformed plantlets.115 transgenic plants were randomly selected to test Hm resistance with leaf segments screening.The results showed that most of transgenic plants tested were Hm resistance,coincident with the results of PCR,indicating that no escapee was existed in the transformants. |
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| Keywords: | Tomato Rice Agrobacterium tumefaciens Gene transfer BADH2 gene |
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