| 长柄扁桃茎尖离体培养研究 |
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| 引用本文： | 孙占育. 长柄扁桃茎尖离体培养研究[J]. 安徽农业科学, 2010, 38(19): 9988-9989. DOI: 10.3969/j.issn.0517-6611.2010.19.023 |
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| 作者姓名： | 孙占育 |
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| 作者单位： | 渭南职业技术学院,陕西渭南,714000 |
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| 摘 要： | [目的]探索长柄扁桃茎尖离体培养技术。[方法]以长柄扁桃试管苗茎尖为材料,接种于QL培养基上,比较不同激素组合对长柄扁桃增殖的影响;将试管苗茎尖接种于不同浓度IBA和1g/L活性炭（AC）的1/2MS培养基暗培养6d,然后转入不含任何激素的1/2MS培养基中,比较不同浓度IBA对长柄扁桃诱导生根的影响。[结果]在QL培养基中分别添加0.2mg/L6-BA和0.6mg/LIBA的增殖效果较好,增殖倍数可达3.96;在1/2MS的培养基中添加30mg/LIBA诱导生根较好,生根率达93.3%。[结论]长柄扁桃试管苗茎尖适宜于增殖的培养基为QL＋6-BA0.2mg/L＋IBA0.6mg/L,试管苗茎尖诱导生根的适宜培养基为1/2MS＋IBA30mg/L＋活性炭1g/L。
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| 关 键 词： | 长柄扁桃 茎尖 离体培养 |
Study on Micro Propagation of Amygdalus pedunculata Pall. Shoot-tips in vitro |
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| Sun Zhanyu. Study on Micro Propagation of Amygdalus pedunculata Pall. Shoot-tips in vitro[J]. Journal of Anhui Agricultural Sciences, 2010, 38(19): 9988-9989. DOI: 10.3969/j.issn.0517-6611.2010.19.023 |
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| Authors: | Sun Zhanyu |
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| Affiliation: | SUN Zhan-yu(Weinan University of Arts and Sciences,Weinan,Shaanxi 714000) |
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| Abstract: | [Objective] This experimentation aimed to explore micro propagation technology of Amygdalus pedunculata Pall.shoot-tips in vitro.[Method] Shoot-tips of Amygdalus pedunculata Pall.were inoculated in QL medium.The effect of different combinations of hormone on the multiplication of Amygdalus pedunculata was compared.Shoot-tips of Amygdalus pedunculata Pall.were inoculated in 1/2MS medium with different concentrations of IBA and 1 g/L AC with 6 d treatment then in 1/2MS medium without any hormone.The effect of... |
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| Keywords: | Amygdalus pedunculata Pall. Shoot-tips Micro propagation |
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