狗枣猕猴桃叶片离体培养的器官、体细胞胚形成与植株再生(英文)

以狗枣猕猴桃试管苗的叶片为外植体,接种于含3%蔗糖和0.2%Gelrite的BW培养基上,外加2,4-D(0,0.1,1和10μmol/L)与玉米素(0,1和10μmol/L)的12种激素组合,置于25℃,光周期为16/8h,光照强度为4000lx的条件下培养。在含1或10μmol/L2,4-D与1或10μmol/L玉米素组合的BW培养基上,产生了体细胞胚,并分化出小植株。随着玉米素浓度的增加,每个外植体上的胚再生频率和体细胞胚的数量也随之增加。同时以叶片为外植体产生的狗枣猕猴桃试管苗的愈伤组织表层产生了不定芽,并抽长成枝。发枝率随着玉米素浓度的增加而增加,并受高浓度的2,4-D所抑制。枝芽转接到含1μmol/LNAA的BW培养基上生根,长成小植株。

Organogenesis, somatic embryogenesis and plantlet regeneration from leaf explants of Actinidia kolomikta cultured in vitro

Leaf explants from Actinidia kolomikta were cultured on BW media containing 30 g/L sucrose and 2 g/L Gelrite and supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Zeatin. Adventitious buds were generated from calli derived from leaf explants and developed into shoots. Shoot regeneration rate and the number of shoots regenerated increased with the increase in Zeatin concentration and decreased with the increase in 2,4-D concentration. Embryogenesis was achieved in the BW medium added with 1 μmol/L each of 2,4-D and Zeatin. Subculture of the embryogenic callus led to secondary embryogenesis with a high frequency of 66.7％. In rooting culture of shoots, roots formed directly from the basal portion of shoots on the medium containing 2,4-D or α-Naphthaleneacetic acid (NAA). Plants were successfully acclimatized to the field conditions with a survival rate of 100％.