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| 槟榔基因组DNA提取方法及RAPD分析体系的初步优化 | |
| 引用本文： | 刘慧娟,车海彦,温衍生,罗大全. 槟榔基因组DNA提取方法及RAPD分析体系的初步优化[J]. 热带作物学报, 2010, 31(7): 1109-1114. DOI: 10.3969/j.issn.1000-2561.2010.07.011 |
| 作者姓名： | 刘慧娟 车海彦 温衍生 罗大全 |
| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737;海南大学环境与植物保护学院,海南,儋州,571737 2. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737;农业部热带农林有害生物入侵监测与控制重点开放实验室,海南,儋州,571737 3. 中国热带农业科学院环境与植物保护研究所,海南,儋州,571737 |
| 基金项目： | 国家自然科学基金项目，国家科技支撑计划项目课题，中央级公益性科研院所基本业务费专项 |
| 摘 要： | 采用CTAB法、改良CTAB法和高盐低pH法等3种不同的方法比较了槟榔基因组DNA提取的效果,其中改良CTAB法最优,提取的DNA质量高,DNA降解少,杂质含量少。通过对退火温度、DNA模板用量、TaqDNA聚合酶浓度等因素的优化实验,建立了槟榔RAPD分析体系,从PCR扩增程序和反应体系两个方面对槟榔RAPD分析体系进行了优化并验证了优化后的体系扩增谱带清晰、多态性强稳定性好。 |
| 关 键 词： | 槟榔 DNA提取 RAPD分析 体系 |
Extraction of DNA and Establishment of RAPD System in Areca |
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| Liu Huijuan,Che Haiyan,Wen Yansheng and Luo Daquan. Extraction of DNA and Establishment of RAPD System in Areca[J]. Chinese Journal of Tropical Crops, 2010, 31(7): 1109-1114. DOI: 10.3969/j.issn.1000-2561.2010.07.011 | |
| Authors: | Liu Huijuan Che Haiyan Wen Yansheng Luo Daquan |
| Affiliation: | 1 Environment and Plant Protection Institute2 College of Environment and Plant Protection, Hainan University;1 Environment and Plant Protection Institute 3 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province;1 Environment and Plant Protection Institute 3 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province;1 Environment and Plant Protection Institute 3 Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agriculture of Hainan Province |
| Abstract: | The three methods for extraction of genomic DNA CTAB method, improved CTAB method and High salts and low pH method are practical and efficient. The result showed that the improved CTAB method is the best method, because it obtains high-quality genomic DNA with less degradation and impurity. A number of factors affected the results of RAPD were studied such as annealing temperature, quantity of template DNA and concentration of Taq DNA polymerase enzyme etc. The optimized RAPD analysis system of the areca was established. Several clear bands with better polymorphism, stability and repeatability could be obtained. |
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