Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows |
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| Authors: | Awad Walid S Ibrahim Adel K Mahran Khaled Fararh Khaled M Abdel Moniem Mervet I |
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| Institution: | (1) Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt;(2) Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;(3) Department of Clinical Pathology, Faculty of Veterinary Medicine, Banha University, Giza, Egypt;(4) Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt |
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| Abstract: | Viral isolation, polymerase chain reaction (PCR), dot blot hybridization (DBH), and indirect enzyme-linked immunosorbent assay
(iELISA) were used for the diagnosis of lumpy skin disease in clinically infected, fevered, and apparently normal dairy cows.
Lumpy skin disease virus (LSDV) was isolated from skin biopsies and blood samples collected from clinically infected cows
in percentages of 72% and 20%, respectively. The virus recovered from blood samples collected from fevered cows in percentage
of 33.3%. Both PCR and DBH detected viral DNA in 100% of skin biopsies collected from clinically infected cows whereas the
detection rates in blood samples collected from clinically infected animals were 100% and 84% using PCR and DBH, respectively.
Viral DNA was detected in blood samples collected from fevered cows using PCR and DBH in percentages of 77.8% and 66.6%, respectively.
Only 19.1% of blood samples collected from in-contact cows was positive for both of PCR and DBH. Detection rates of antibodies
against LSDV using iELISA in serum samples collected from clinically infected and fevered cows were 56% and 11.1%, respectively,
whereas all in-contact cows had no antibodies against the virus. |
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