2,4-D和NAA对成熟玉米种子不同外植体胚性愈伤组织诱导效果的影响

以成熟玉米K12种子的胚、胚根、胚轴为外植体材料,研究了2,4-D和NAA及其浓度配比对愈伤组织诱导效果的影响.结果表明:不同外植体对激素浓度的要求不同,只用1种激素处理时,2,4-D的浓度以2.0~2.5mg/L为宜,NAA浓度以0.5~0.6 mg/L为宜;2种激素配比使用时,以2,4-D 2.0~2.5mg/L与NAA 0.6 mg/L浓度配比效果较佳,且其效果好于单一激素处理.配合使用比单独使用具有更好的诱导效果.

Influence of 2,4-D and NAA on embryonic callus induction from different explants in mature seeds of the maize parent K12

The embryo,radicle and hypocotyl in mature seeds of the maize parent K12 were used as explants to study the influence of two exogenous hormone such as 2,4-D,NAA as well as the concentration ratio between them on embryonic callus induction.The results showed that the callus induction from different embryonic explants of maize required different concentration of 2,4-D and NAA.The 2,4-D concentration resulting the best induction effect was determined as 2.0～2.5 mg/L,and the NAA concentration with the best induction effect was as 0.5～0.6 mg/L.The combination treatments with 2,4-D and NAA had better effect than single hormone treatments,among which the best concentration combination was as 2,4-D 2.0～2.5 mg/L with NAA 0.6 mg/L.