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TPM1基因在猪背最长肌生长发育过程中的动态表达
引用本文:梁如意,李岩,唐中林,黄瑞华,李奎.TPM1基因在猪背最长肌生长发育过程中的动态表达[J].中国农业科学,2012,45(11):2267-2272.
作者姓名:梁如意  李岩  唐中林  黄瑞华  李奎
作者单位:1.南京农业大学动物科技学院,南京210095 2.中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与利用重点开放实验室,北京 100193
基金项目:国家自然科学基金项目(30830080);国家“973”计划项目(2009CB941604);江苏省科技支撑计划(BE2009330-1,BE2008390)
摘    要:【目的】探究原肌球蛋白(tropomyosin,TM)是否在猪背最长肌生长发育过程中发挥生物学功能。【方法】采用SYBR® Green II 荧光法进行荧光定量PCR分析TPM1基因在通城猪和长白猪中的组织表达谱,以及出生前和出生后背最长肌发育中(28个发育点)的表达变化。【结果】TPM1基因在两种猪中背肌和心肌中都有丰富地表达,在背最长肌发育过程中的表达趋势相似,即TPM1基因的表达量先增加后减少,但出现峰值的时间不同(通城猪中,TPM1基因在胚胎期75 d的表达量显著高于其它时间点,P<0.05;长白猪中,TPM1基因在胚胎期90 d的表达量显著高于其它时间点,P<0.05)。【结论】TPM1基因的表达与猪背最长肌生长发育及品种间肌肉表型差异有关。

关 键 词:TPM1基因  荧光定量PCR  背最长肌发育  动态表达  猪   
收稿时间:2011-12-29

Dynamic Expression of TPM1 Gene During Longissimus dorsi Muscle Development in Pigs
LIANG Ru-yi , LI Yan , TANG Zhong-lin , HUANG Rui-hua , LI Kui.Dynamic Expression of TPM1 Gene During Longissimus dorsi Muscle Development in Pigs[J].Scientia Agricultura Sinica,2012,45(11):2267-2272.
Authors:LIANG Ru-yi  LI Yan  TANG Zhong-lin  HUANG Rui-hua  LI Kui
Institution:1College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095;2Institute of Animal Science,Chinese Academy of Agricultural Sciences/State Key Laboratory of Farm Animal Genetic Resources and Utilization,Beijing 100193)
Abstract:【Objective】The purpose of the present research was to explore whether tropomyosin(TM) has biological functions in the regulation of the Longissimus dorsi muscle growth and development of pigs.【Method】In this study,TPM1 gene expression profiles in different tissues from Tongcheng pigs and Landrace pigs were analyzed and the expression change during Longissimus dorsi muscle development(28 stages) was investigated by real-time qPCR with SYBR Green Ⅱ.【Result】The result showed that the TPM1 gene was strongly expressed in the Longissimus dorsi muscle and heart,and both breeds were similar in the expression patterns with decreasing after increasing,but the peak occurred at different times.The expression level of TPM1 gene in embryonic period 75 d were significantly higher than those in other periods in Tongcheng pigs,and the same result was found in embryonic period 90 d in Landrace pigs.【Conclusion】Results suggested that TPM1 gene expression may be related to the Longissimus dorsi muscle growth and muscle phenotypic differences between species of pigs.
Keywords:TPM1 gene  RT-qPCR  Longissimus dorsi muscle development  dynamic expression  pig
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