白腐菌发酵玉米秸秆最佳条件研究

【目的】以玉米秸秆为材料，研究白腐菌对其发酵的最佳条件。【方法】采用L₉（3⁴）正交试验设计，以发酵后玉米秸秆中的粗蛋白和粗纤维含量为指标，以白腐菌发酵玉米秸秆时的秸秆含水率、温度、接种量、起始pH为主要影响因素，筛选白腐菌发酵玉米秸秆的最佳条件。【结果】白腐菌发酵玉米秸秆的最优条件为：秸秆含水率为70%，温度为30 ℃，接种量为0.25 g/kg，起始pH为4，在此条件下发酵7 d，秸秆粗纤维含量由348.7 g/kg下降为299.4 g/kg，粗蛋白含量由58.3 g/kg升高为116.6 g/kg。【结论】筛选出了白腐菌发酵玉米秸秆的最佳条件，为玉米秸秆资源化利用提供了参考。

Fermentation conditions of optimization white-rot fungi on corn straw stem

【Objective】 Crude protein and crude fiber content were determined at the beginning and the end of 7,12,17-days fermentation of white-rot fungi on corn straw in order to obtain optimized condition of fermentation,such as the optimal time,temperature,acidity,inoculum concentration and water content of corn straw.【Method】 In order to select the optimized fermentative conditions of white-rot fungi on corn stem,L9(34) orthogonal experiment was designed in this trial,crude protein and crude fibre content of corn were determined,water ratio,temperature,inoculum concentration and acidity in the fermentation of white-rot fungi on corn stem were designed as main influencing factors.【Result】 Optimized condition of fermentation of white-rot on corn straw was:water ratio 70%,temperature 30 ℃,inoculum concentration 0.25 g/kg corn straw and pH 4 during 7 days.Under above optimizing fermentative condition,crude fiber content was decreased from 348.7 g/kg to 299.4 g/kg,crude protein content was increased from 58.3 g/kg to 116.6 g/kg.【Conclusion】 Optimized fermentative conditions of white-rot fungi on corn stem were selected,which would provide some reference in utilizing the corn stem as feed resource.