| 五种常用的植物转基因技术 |
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| 引用本文： | 汪由,吴禹,王岩,李兆波,王光霞.五种常用的植物转基因技术[J].杂粮作物,2010,30(3):186-189. |
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| 作者姓名： | 汪由 吴禹 王岩 李兆波 王光霞 |
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| 作者单位： | 1. 辽宁省农业科学院,创新中心,辽宁,沈阳,110161
2. 沈阳市东陵区白塔街道办事处,辽宁,沈阳,110167 |
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| 摘 要： | 从原理、基本步骤和优缺点等几个方面对农杆菌介导法、基因枪法、超声波介导法、子房注射法和花粉管通道法等5种常用的植物转基因技术进行了简要介绍。
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| 关 键 词： | 农杆菌介导法 基因枪法 超声波介导法 子房注射法 花粉管通道法 原理 基本步骤 优缺点 |
The Five Kinds of DNA Transformation Techniques |
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| WANG You,WU Yu,WANG Yan,LI Zhao-bo,WANG Guang-xia.The Five Kinds of DNA Transformation Techniques[J].Rain Fed Crops,2010,30(3):186-189. |
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| Authors: | WANG You WU Yu WANG Yan LI Zhao-bo WANG Guang-xia |
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| Institution: | 1 Innovation Center of Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning,110161;2 Baita Subdistrict Office of Dongling District of Shenyang,Shenyang,Liaoning,110167,China) |
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| Abstract: | In this paper,a brief introduction about the principle、basic method and advantages and disadvantages of the five DNA transformation techniques： Agrobacterium-mediated transformation、gene gun bombardment、ultrasound-mediated transformation、ovary injection transformation and pollen-tube pathway transformation has been reviewed. |
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| Keywords: | agrobacterium-mediated transformation gene gun bombardment ultrasound-mediated transformation ovary injection transformation pollen-tube pathway transformation principle basic method advantages and disadvantages |
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