| HPLC测定宫衣净酊中葛根素含量 |
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| 引用本文： | 朱永刚,崔东安,王磊,王旭荣,张景艳,林杰,杨志强,李建喜. HPLC测定宫衣净酊中葛根素含量[J]. 动物医学进展, 2016, 0(4): 74-76. DOI: 10.3969/j.issn.1007-5038.2016.04.016 |
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| 作者姓名： | 朱永刚 崔东安 王磊 王旭荣 张景艳 林杰 杨志强 李建喜 |
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| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所/甘肃省中兽药工程技术研究中心,甘肃兰州,730050 |
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| 基金项目： | 公益性行业(农业)科研专项项目(201303040) |
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| 摘 要： | 为了建立宫衣净酊中葛根素的高效液相色谱(HPLC)测定方法,应用色谱柱为安捷伦Eclipse XDB-C18(4.6mm×250mm,5μm),流动相为甲醇-水(25∶75),流速为1mL/min,检测波长为250nm,柱温为30℃。结果表明,在此色谱条件下,葛根素的线性范围为18.25μg/mL~584.00μg/mL(r=0.999 9,n=6),平均回收率为108.83%(RSD=1.03%)。说明本方法简便、准确、重现性好,可以作为宫衣净酊的质量控制方法。
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| 关 键 词： | 高效液相色谱 宫衣净酊 葛根素 含量测定 |
Determination of Puerarin Content in Gong-Yi-Jing Tincture by HPLC |
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| Abstract: | In order to establish an HPLC method for the determination of puerarin in Gong-Yi-Jing tincture, the Agilent Eclipse XDB-C18 column(4.6 mm× 250 mm,5 μm),the mobile phase of methanol to water (25∶75),flow rate of 1 mL/min were used.The column temperature is 30℃.The results showed that,in this chromatographic conditions,the liner range of puerarin was 18.25-584.00μg/mL(r=0.999 9,n=6). The average recovery rate was 108.83%(r=1.76%,n=6).The system precision is 1.025%.The results illustrated that the HPLC method is simple,accurate,repeatable and can be used for quality control of Gong-Yi-Jing tincture. |
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| Keywords: | HPLC Gong-Yi-Jing tincture puerarin content determination |
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