Characteristics of two major lectins from mungbean (Vigna radiata) seeds |
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| Authors: | K N Suseelan C R Bhatia R Mitra |
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| Institution: | (1) Nuclear Agriculture Division, Bhabha Atomic Research Centre, 400 085 Mumbai, India;(2) 17, Rohini Cooperative Housing Society, Sector 9A, Vashi, Navi Mumbai, India |
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| Abstract: | Two major lectins, MBL-I and MBL-II, were purified fromVigna radiata L. seeds using ion-exchange and gel filtration chromatography techniques. MBL-I was found to be a tetramer with native M.W.
of 132 kDa and subunit M.W. of 33 kDa having α-galactosidase activity. MBL-II consisted of two monomeric lectins with M.W.
of 94 kDa and 89 kDa which were associated mainly with β-galactosidase activity. Both MBL-I and MBL-II are D-galactose-specific
lectins. |
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| Keywords: | α -Galactosidase β -Galactosidase Greengram Lectins Monomeric Mungbean |
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