| 苏云金芽孢杆菌Cry1Ac杀虫晶体蛋白及其分子设计 |
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| 引用本文： | 刘卓明,谢柳,叶大维.苏云金芽孢杆菌Cry1Ac杀虫晶体蛋白及其分子设计[J].广西农业生物科学,2009(2):356-364. |
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| 作者姓名： | 刘卓明 谢柳 叶大维 |
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| 作者单位： | 广西大学生命科学与技术学院;海南海德热带农业资源研究所;南开大学生命科学院; |
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| 基金项目： | 中国Bt资源收鉴与利用项目;;国家863项目(2006AA022189)资助 |
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| 摘 要： | Cry1Ac编码的杀虫晶体蛋白是苏云金芽孢杆菌（Bt）产生的多种杀虫晶体蛋白中对鳞翅目昆虫有很高毒性的蛋白。第一个Cry1Ac杀虫晶体蛋白最早在库斯塔克亚种HD73中以伴胞晶体形式分离获得，其编码区为3534bp，编码蛋白分子量为133kD，含1178个氨基酸，等电点为4.84。白此以来，Cry1Ac杀虫晶体蛋白结构、功能以及应用研究一直是Bt杀虫晶体蛋白研究的重要方向。本文介绍了苏云金芽孢杆菌中应用最广泛的Cry1Ac杀虫晶体蛋白家族的结构、功能及其基因分类，并进一步就基于苏云金芽孢杆菌Cry1Ac杀虫晶体蛋白的基因工程研究做了分析，提出了持续利用Bt Cry1Ac杀虫晶体蛋白的一些见解。
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| 关 键 词： | 苏云金芽孢杆菌 Cry1Ac杀虫晶体蛋白 分子设计 |
Bt Cry1Ac Insecticidal Crystal Protein Family and its Molecular Design |
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| Liu Zhuoming , Xie Liu , Ye Dawei College of Life , Technology Science,Guangxi University,Nanning, College of Life Sciences,Nankai University,Tianjin, Haide Institute of Tropical Agricultural Resources,Sanya.Bt Cry1Ac Insecticidal Crystal Protein Family and its Molecular Design[J].Journal of Guangxi Agricultural and Biological Science,2009(2):356-364. |
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| Authors: | Liu Zhuoming Xie Liu Ye Dawei College of Life Technology Science Guangxi University Nanning College of Life Sciences Nankai University Tianjin Haide Institute of Tropical Agricultural Resources Sanya |
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| Institution: | Liu Zhuoming 1,3 Xie Liu 1,3 Ye Dawei 2 1 College of Life , Technology Science,Guangxi University,Nanning,530005,2 College of Life Sciences,Nankai University,Tianjin,300071,3 Haide Institute of Tropical Agricultural Resources,Sanya,572025 |
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| Abstract: | Bacillus thuringiensis insecticidal crystal protein has exhibited high toxicity to some agricultural in-sects. Cry1Ac insecticidal crystal proteins were firstly obtained from the Bt kurstaki HD73 in the form of paraspo-ral crystals,the open reading frame of the cry1Ac gene contains 3 534 bp encoding a 133 kD protein with 1 178 amino acids and its IE point was 4.84. This paper summarizes the structure,function and gene taxonomy of Cry1Ac insecticidal crystal proteins and reviews as well the research of genet... |
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| Keywords: | Bacillus thuringiensis Cry1Ac insecticidal crystal protein Molecular design |
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