| 基于均匀设计法优化毛毡杜鹃高效快繁体系 |
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| 引用本文： | 顾地周,高捍东,曹逊,马秋月,顾美影,朱俊义.基于均匀设计法优化毛毡杜鹃高效快繁体系[J].林业科技,2009,34(2):57-59. |
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| 作者姓名： | 顾地周 高捍东 曹逊 马秋月 顾美影 朱俊义 |
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| 作者单位： | 1. 通化师范学院生物系,吉林,通化,134002
2. 南京林业大学森林资源与环境学院,江苏,南京,210037 |
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| 基金项目： | 吉林省科技厅资助项目，通化师范学院自然科学基金 |
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| 摘 要： | 以毛毡杜鹃嫩茎段为外植体，应用均匀设计法筛选最适合的培养基。试验结果表明，适合嫩茎段腋芽萌发生长及生根的最佳培养基为：MS（改良）＋IAA 0．10mg/L＋IBA 0．30mg／L＋NAA 0．12mg／L＋GA 31．80mg／L，再生率达93％以上。以再生植株的茎节为材料进行快繁的结果表明，在25天的一个培养周期内，茎段的增殖倍数平均达50以上。
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| 关 键 词： | 毛毡杜鹃 组织培养 快繁 均匀设计 |
Uniform Design for High Efficient Micropropagation System of Rhododendron confertissimum |
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| GU Dizhou.Uniform Design for High Efficient Micropropagation System of Rhododendron confertissimum[J].Forestry Science & Technology,2009,34(2):57-59. |
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| Authors: | GU Dizhou |
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| Institution: | Tonghua Normal University;Jilin Tonghua 134002 |
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| Abstract: | Take young stems of Rhododendron confertissimum as explants and suitable medium compositions for axillary bud growing and rooting were screened through uniform design experiments.The results showed that MS(modified)+IAA0.10 mg/L+IBA0.30 mg/L+NAA0.12 mg/L+GA31.80 mg/L was the most suitable with a regeneration rate of 93%.Stems each with one node were cut from regenerated shoots and cultured for propagation,and a 50-fold proliferation rate of stems was achieved within 25 days. |
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| Keywords: | Rhododendron confertissimum Tissue culture Micropropagation Uniform design |
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