| 利用SSR技术鉴定苏玉20的纯度 |
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| 引用本文： | 孟庆长,陈艳萍,杨兴华,赵文明,张彦兵,袁建华.利用SSR技术鉴定苏玉20的纯度[J].江苏农业学报,2009,25(3). |
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| 作者姓名： | 孟庆长 陈艳萍 杨兴华 赵文明 张彦兵 袁建华 |
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| 作者单位： | 1. 江苏省农业科学院粮食作物研究所,江苏南京,210014
2. 江苏宿迁中江种业有限公司,江苏宿迁,223800 |
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| 基金项目： | 国家科技支撑计划，国家农业科技成果转化资金项目，江苏省农业科技自主创新资金项目 |
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| 摘 要： | 苏玉20是江苏省农业科学院2004年选育成功的高产优质玉米杂交种.以苏玉20玉米杂交种及其亲本为材料,从80对玉米SSR引物中筛选出在苏玉20双亲之间多态性明显、重复性较好的4对引物bnlg1306、phi065、bnlg2291和umc1590用于苏玉20纯度鉴定.这4个SSR标记特异性好,且人为掺杂与田间鉴定均与利用SSR标记鉴定结果高度一致.尤其是,这些引物同时可以用于郑单958的纯度鉴定并可以区分苏玉20和郑单958杂交种.因此,该方法具有准确可靠、实用性强等优点,为生产上杂交种苏玉20纯度的快捷有效鉴定提供了科学依据.
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| 关 键 词： | 玉米 杂交种 SSR标记 纯度鉴定 |
Seed Purity Test for Maize Hybrid Suyu 20 Using SSR Markers |
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| MENG Qing-chang,CHEN Yan-ping,YANG Xing-hua,ZHAO Wen-ming,ZHANG Yan-bing,YUAN Jian-hua.Seed Purity Test for Maize Hybrid Suyu 20 Using SSR Markers[J].Jiangsu Journal of Agricultural Sciences,2009,25(3). |
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| Authors: | MENG Qing-chang CHEN Yan-ping YANG Xing-hua ZHAO Wen-ming ZHANG Yan-bing YUAN Jian-hua |
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| Institution: | 1.Institute of Food Crops;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China;2.Jiangsu Zhongjiang Seed Industry Co.Ltd;Suqian 223800;China |
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| Abstract: | Maize hybrid Suyu 20 is a super cultivar with high yield and good quality bred by Jiangsu Academy of Agricultural Sciences in 2004.Genomic DNA extracted from Suyu 20 and its parents were subjected to SSR analysis with 80 pairs of SSR primers.As a result,four pairs of primers bnlg1306,phi065,bnlg2291 and umc1590 which could detect polymorphism between the parents were determined as candidates for purity assessment of Suyu 20.The purity test using the four specific markers was in agreement with the detection ... |
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