药用野生稻抗稻褐飞虱鉴定与利用技术研究

从198份药用野生稻资源中筛选出一批广谱高抗褐飞虱抗源（其中3份为免疫级）并对这些抗源进行了抗性遗传研究。研究结果表明其抗性是受一对显性基因控制的。通过离体幼胚培养获得绿苗，经过多代回交和自交，成功地将抗性基因转移到栽培稻中，获得了高世代（B4F5)株系。同时利用外源DNA花粉管导入法对药用野生稻广谱高抗褐飞虱基因导入到栽培稻进行了研究，获得了具有某些性状的后代。

Technique for Utilization and Identification of Resistance to Brown Planthopper in Oryza of ficinalis

Three immune and 89 highly resistant wild rice ma te rials to brown planthopper were selected from 198 tested collections of O.of ficinalis. Genetic analysis indicated that the inheritance to brown plantho pper was conditioned by a pair of dominant gene. When Oryza officinalis with high resistance to brown planthopper was crossed with O. s ativa by routine breeding and biotechnique with different hybridizat ion ways and varieties, F 1 hybrids could be achieved by embryo rescue tec hnique, and the resistance gene of O. officinalis was introgres sed into O. sativa by backcrossing and inbred. Exogenous DNA of the resistance of O. officinalis could be also introduced into O. sativa by the technique of pollen tube passage way, and some offsprin gs were obtained.