橙色大白菜遗传转化体系的初步建立

以06J28橙色大白菜子叶段为外植体,通过根癌农杆菌介导CMS7311-orf224基因,探讨了潮霉素、头孢霉素、预培养时间、农杆菌浓度、感菌时间和共培养时间等因素对橙色大白菜遗传转化的影响.试验结果表明,子叶段预培养2~3 d后,在OD600值为0.3~0.5的农杆菌EHA-105菌液中侵染5 min,再共培养2~3 d,在培养基中加入5 mg/L Hyg (抗性筛选)和500 mg/L Cef(脱菌)可得到转化植株.试验初步建立了橙色大白菜遗传转化体系,为大白菜种质资源创新奠定了基础.

Establishment of Genetic Transformation System for Orange Heading Chinese Cabbage

In this study,several factors affecting the transformation rate were studied by Agrobacterium-mediated CMS7311-orf224 gene in 06J28 Chinese cabbage,and these factors included sensitivity of cotyledon segments to hygromycin（Hyg） and Cefotaxime（Cef）,pre-culture days,concentrations of Agrobacterium tumefaciens,co-culture days and times of being infected on the optimum culture medium.The results showed that,resistant plants could be obtained with the conditions as follows： the cotyledon segments of 06J28 Chinese cabbage were pre-cultured for 2-3 days,and then infected with EHA-105（the OD600 value ranged from 0.3 to 0.5） for 5 minutes and were co-cultured for 2-3 days,lastly were transformed to screening-culture medium（adding 5 mg/L Hyg and 500 mg/L Cef）.A higher efficient genetic transformation system was established for orange Chinese cabbage,which set up a basis for germplasm resources innovation of Chinese cabbage.