围小丛壳菌山茶专化型与葡萄座腔菌复合侵染引致茶褐枯病

在山东日照东港区茶园茶树叶部发现一种新病害。感病部位缢缩,畸形,病斑褐色,坏死,命名为茶褐枯病。取病健交界处采用组织分离法共获得两种微生物,编号为RC4、RC3,分离率分别为67.1%和32.9%。结合微生物形态学、培养学、多基因序列分析和柯赫氏法则验证,明确该病害由围小丛壳菌山茶专化型（Glomerella cingulata f. sp. camelliae）和葡萄座腔菌（Botryosphaeria dothidea）复合侵染引起。这也是首次在茶树叶部发现B. dothidea的危害。

The Tea Brown Blight Disease Caused by Co-infection of Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea

s: A new leaf disease of tea plant was found in Donggang district of Rizhao, Shandong Province. The infected parts were shrunken and malformed, turned brown, necrotic and named brown blight of tea. Two microorganisms were isolated from the edge of infected parts through tissue separation, and named as RC4 and RC3. Their separation rates were 67.1% and 32.9% respectively. According to morphology, culture characteristics, multi-sequence analysis and Koch's Postulates test, Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea were found to be the cause of disease. This was the first report that Botryosphaeria dothidea damaged the leaves of tea plant.