扁桃SLF基因和S-RNase基因的克隆及表达分析

 以扁桃‘Pioneer’品种为材料, 利用RT-PCR及RACE技术, 克隆了1个新的SLF ( S LocusF-box ) 基因(PdSLF1) 和两个新的S-RNase基因( PdSm 和PdSn) 的cDNA。PdSLF1全长1 331 bp, 编码376个氨基酸; PdSm 全长826 bp, 编码228个氨基酸; PdSn基因全长878 bp, 编码227个氨基酸。与公共数据库中的序列进行相似性比较, 发现这3个基因所编码的氨基酸序列与其它蔷薇科植物相应基因的氨基酸序列均具有较高的一致性, PdSLF1为70.2% ～84.8% , S-RNase为59% ～83.9%。PdSLF1基因在花药中专一性表达, 而PdSm 和PdSn基因在雌蕊中专一性表达。

Cloning and Expression Analysis of the SLF and S-RNase Genes in Almond

Using RT-PCR and Rapid Amplification of cDNA Ends(RACE)techniques,cDNAs for one novel SLF(S Locus F-box)(PdSLF1)gene and two novel alleles of S-RNase genes(PdSm and PdSn)were cloned from almond(Prunus dulcis Mill.)cultivar 'Pioneer',and their expression patterns were investigated in anthers,pistils,petals,sepals and leaves.PdSLF1 was 1 331 bp in length encoding a protein of 376 amino acids.The complete cDNA of PdSm was 826 bp encoding 228 amino acids and PdSn 878 bp encoding 227 amino acids.Compared the deduced protein sequences with those registered in GenBank,PdSLF1,PdSm and PdSn proteins shared high identities with other SLF(70.2%-84.8%)and S-RNase(59%-83.9%)proteins,respectively.Furthermore,the results of semi-quantitative RT-PCR analysis showed that PdSLF1 expressed exclusively in anthers,and two alleles of S-RNase,PdSm and PdSn,exclusively in pistils,which suggested that they may participate in self-incompatibility in almond.