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欧鳗脾和肾中黑色素巨噬细胞中心的形态学与细胞化学研究
基金项目:福建省自然科学基金项目(2012J01066)
摘    要:应用组织化学与电镜技术对欧鳗脾脏和肾脏中黑色素巨噬细胞中心(MMCs)进行研究。结果表明:脾脏和肾脏中均存在MMCs,它们呈圆形、卵圆形或不规则形状;脾脏MMCs有邻血管分布的特点,而肾脏MMCs的分布无此特点;脾脏和肾脏中的MMCs均含有铁血黄素、黑色素及脂褐质3种色素,但脾脏MMCs以铁血黄素为主,肾脏以黑色素和脂褐质为主;脾脏MMCs中存在色素颗粒和细胞碎片。初步推测,欧鳗脾脏MMCs与肾脏MMCs的功能有可能是不相同的。

关 键 词:欧鳗  黑色素巨噬细胞中心  形态学  细胞化学  脾脏  肾脏

Morphology and cytochemistry of melano-macrophage centers in spleen and kidney of Anguilla anguilla
Abstract:The morphology and cytochemistry of melano-macrophage centers (MMCs) in spleen and kidney of eel (Anguilla anguilla) were assessed by employing technologies of histochemistry and transmission electron microscopy. The results showed that there were MMCs both in spleen and kidney with round, oval or irregular shape; the majority of MMCs in spleen distributed regularly around blood vessel, on the contrary, MMCs in kidney did not follow the rule; the MMCs both in spleen and kidney were contained with haemosiderin, melanin and lipofuscin, while, haemosiderin dominated in the former and melanin and lipofuscin did in the latter. Ultrastructural observations revealed that there were granular pigments and cellular debrises in MMCs of spleen. It seemed that MMCs in spleen and kidney undertook different function inferred from the experiments.
Keywords:Anguilla anguill  melano-macrophage centers  morphology  cytochemistry  spleen  kidney
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