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A Cucumber mosaic virus isolated from Momordica charantia L.
Authors:Keiji Takami  Hiroyuki Okubo  Shuichi Yamasaki  Minoru Takeshita  Yoichi Takanami
Affiliation:(1) Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;(2) Oita Prefectural Agriculture, Forestry, and Fisheries Research Center, Oita, Japan
Abstract:Momordica charantia L. plants systemically infected with Cucumber mosaic virus (CMV) were found in Oita Prefecture. The virus isolated from the host plant was characterized by biological, serological, and molecular biological methods. The purified virus was used to mechanically inoculate the host and produced green mottle, green mosaic, and/or chlorotic spots in the noninoculated upper leaves of the host. The virus was identified as an isolate of CMV containing genomic RNA3 derived from subgroup IA by several lines of evidence based on electron microscopy, serological detection, host range, symptoms, and the entire nucleotide sequence of RNA3.
Keywords:Cucumber mosaic virus    Momordica charantia L.   Lagenaria siceraria L.
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