| 蒙古黄芪的组织培养及辐射诱变研究 |
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| 引用本文： | 赵秀娟,张红霞. 蒙古黄芪的组织培养及辐射诱变研究[J]. 安徽农业科学, 2009, 37(9): 3939-3941 |
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| 作者姓名： | 赵秀娟 张红霞 |
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| 作者单位： | 内蒙古科技大学生物与化学工程学院,内蒙古包头,014010;内蒙古科技大学生物与化学工程学院,内蒙古包头,014010 |
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| 基金项目： | 内蒙古科技大学校内基金项目(KJ200611) |
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| 摘 要： | [目的]研究蒙古黄芪的组织培养技术及辐射诱变对愈伤组织诱导的影响。[方法]以蒙古黄芪无菌苗的不同外植体（叶片、子叶、下胚轴）进行愈伤组织诱导,并对子叶和下胚轴进行辐射诱变研究。[结果]3种外植体分别在MS＋6-BA 2.0 mg/L＋NAA 2.0 mg/L、LS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L、MS＋6-BA 2.0 mg/L＋NAA 2.0 mg/L培养基上的愈伤组织诱导率较高 下胚轴和子叶的最佳诱变时间均为15 min 随辐射诱变时间的增加,下胚轴愈伤组织的长势增强,但超过一定的诱变时间愈伤组织长势减弱且诱导率降低。[结论]该研究为蒙古黄芪的组织培养和诱变育种提供科学依据。
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| 关 键 词： | 蒙古黄芪 组织培养 辐射诱变 |
Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao |
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| ZHAO Xiu-juan et al. Study on Tissue Culture and Radiation Mutation of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao[J]. Journal of Anhui Agricultural Sciences, 2009, 37(9): 3939-3941 |
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| Authors: | ZHAO Xiu-juan et al |
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| Affiliation: | ZHAO Xiu-juan et al (Biological , Chemical Engineering School,Inner Mongolia University of Science , Technology,Baotou,Inner Mongolia 014010) |
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| Abstract: | [Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao.[Method] With the different parts of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao aseptic seedling as explants(leaves,cotyledons,hypocotyls) induced callus,and cotyledon and hypocotyls taken by the method of radiation mutation were studied.[Result]The results showed that the three explants had relatively high callus induced rat... |
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| Keywords: | Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao Tissue culture Radiation mutation |
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