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| 腺相关病毒介导的小鼠prnp基因打靶载体构建 | |
| 引用本文： | 王海鹰,万家余,聂代邦,高宏伟.腺相关病毒介导的小鼠prnp基因打靶载体构建[J].江西农业学报,2008,20(5):78-81. |
| 作者姓名： | 王海鹰 万家余 聂代邦 高宏伟 |
| 作者单位： | 1. 军事医学科学院,军事兽医研究所,吉林,长春,130062;吉林大学和平校区,畜牧兽医学院,吉林,长春,130062 2. 军事医学科学院,军事兽医研究所,吉林,长春,130062 3. 吉林大学和平校区,畜牧兽医学院,吉林,长春,130062 |
| 摘 要： | 利用三重融合PCR方法构建了小鼠prnp基因敲除载体。提取129小鼠基因组,设计引物扩增打靶载体同源臂,把两同源臂与neo基因三元件PCR扩增成一条融合基因。融合基因与AAV载体酶切后的载体骨架通过NotI酶切位点相连接,构建重组质粒载体rAAV。 |
| 关 键 词： | Prnp 基因敲除 腺相关病毒载体 融合PCR |
Construction of Adeno-associated Virus Vector for Mouse prnp Gene Targeting |
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| WANG Hai-ying,WAN Jia-yu,NIE Dai-bang,GAO Hong-wei.Construction of Adeno-associated Virus Vector for Mouse prnp Gene Targeting[J].Acta Agriculturae Jiangxi,2008,20(5):78-81. | |
| Authors: | WANG Hai-ying WAN Jia-yu NIE Dai-bang GAO Hong-wei |
| Institution: | WANG Hai - ying, WAN Jia - yu , NIE Dai - bang, GAO Hong - wei (1. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130062, China; 2. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062 ,China) |
| Abstract: | The adeno-associated virus vector for mouse prnp gene targeting was constructed by using three-way fusion PCR method.129 mouse genomes were extracted,the primer for amplification of homology arms was designed,and then the left homology arm,right homology arm and neo gene were fused to one gene.The recombination plasmid vector rAAV was constructed by ligating fusion gene into AAV vector backbone through NotI restriction enzyme site. |
| Keywords: | prnp Gene knockout Adeno-associated virus vector Fusion PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |