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| 高效液相色谱法测定犊泻康颗粒中盐酸小檗碱的含量 | |
| 引用本文： | 阮洪生,吕信,赵乃欢,张会洁,林春驿.高效液相色谱法测定犊泻康颗粒中盐酸小檗碱的含量[J].黑龙江八一农垦大学学报,2009,21(2):54-56. |
| 作者姓名： | 阮洪生 吕信 赵乃欢 张会洁 林春驿 |
| 作者单位： | 1. 黑龙江八一农垦大学生命科学技术学院,大庆,163319 2. 黑龙江八一农垦大学动物科技学院 3. 北京市石景山区爱屋动物医院 |
| 基金项目： | 大庆市科学技术计划项目 |
| 摘 要： | 目的：建立以高效液相色谱法测定犊泻康颗粒中盐酸小檗碱含量的方法。方法：色谱柱为十八烷基键合硅胶柱,流动相：0.033mol/lKH2PO4-乙腈（70：30）,流速：1.0ml/min,检测波长：265nm。结果：在0.40-2.00μg检测范围之间,对照品微克数与峰面积呈良好的线性关系,回归方程Y=3925247x-34180、r=0.9999。平均回收率为99.93%,RSD=0.32%。结论：该方法操作简便、快速、准确,可用于犊泻康颗粒中盐酸小檗碱的含量控制。 |
| 关 键 词： | 高效液相色谱法 犊泻康颗粒 盐酸小檗碱 含量测定 |
Determination of Berberine Hydrochloride in Duxiekang Granules by HPLC |
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| Ruan Hongsheng,Lv Xin,Zhao Naihuan,Zhang Huijie,Lin Chunyi.Determination of Berberine Hydrochloride in Duxiekang Granules by HPLC[J].Journal of Heilongjiang August First Land Reclamation University,2009,21(2):54-56. | |
| Authors: | Ruan Hongsheng Lv Xin Zhao Naihuan Zhang Huijie Lin Chunyi |
| Institution: | Ruan Hongsheng, Lv Xin, Zhao Naihuan, Zhang Huijie, Lin Chunyi (1. College of Life Science and Technology, Heilongjiang August First Land Reclamation University, Daqing, 163319; 2. College of Animal Science and Technology, Heilongjiang August First Land Reclamation University; 3. Ai Wu Veterinary hospital Beijing Shijing Hill area ) |
| Abstract: | Objective ： to establish the determination of Berbefine Hydrochloride in Duxiekang Granules, HPLC was adopted. Methods： In this method ODS C18 column was used , 0.033 mol/l KH2PO4- acetonitrile （70：30） was used as a mobile phase. The detection wave-length was at 265nm.Restults： The detection range is 0.40-2.00 μg.There is agood linear relationship between consistence and apex-area. The regress equationis Y=3 925 247x-34 180,r=0.999 9. The recovery of the added sample was 99.93%,And RSD：0.32%.Conclusion： This method was simple and the result was reliable. |
| Keywords: | HPLC Duxiekang Granules Berberine Hydrochloride determination |
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