矮生福禄考组织培养快速繁殖的研究

为满足栽培对种苗的需求，以矮生福禄考有芽嫩茎为材料，进行了芽的生长、生长芽分化、不定芽生根、试管苗的生根继代，以及试管苗的移栽和定植的研究，建立了矮生福禄考快速繁殖技术。结果表明：1／3MS或1／2MS＋IBA0．1mg·L^-1＋IAA0．2mg·L^-1是矮生福禄考芽生长的理想培养基；MS＋AgNO31．0mg·L^-1＋6BA0．8mg·L-1。＋NAA0．1mg·L^-1是矮生福禄考生长芽分化培养的理想培养基；用浓度为20mg·L^-1的NAA溶液对不定芽基部处理5min后，接种到white＋IAA0．4mg·L^-1的培养基上生根的方法是矮生福禄考不定芽生根培养和试管苗生根继代培养的理想方法。移栽试管苗容易成活，定植的试管苗出现了生长旺盛、根系增加1倍左右、平均花期延长7d、秋末冬初枯萎晚10d左右的特点。

Study on Rapid Propagation Tissue Culture of Phlox Drummondii Var Nana

In order to meet the need of cultivation of germchits, the tender stems with buds of Phlox drummondii varnana are used as materials to make the research on bud growth and differentiation, rooting of adventitious buds, rooting and transplanting of tube seedlings, finally, the rapid propagation techniques of Phlox drummondii var nana is established. The results show that 1/3MS-1/2 MS＋IBA 0. 1 mg· L^-1＋ IAA0.2mg ·L-1 is the optimum medium for buds growth,and the optimum medium for buds differentiation is MS＋AgNO31. 0mg · L-1 ＋6-BA 0. 8mg · L-1 ＋NAA 0. 1 mg ·L 1. After treatment with IAA （concentration of 20mg ·L.-1） for 5 minutes,the adventitious buds are easily induced rooting in the medium of White-IAA0.4mg · L ,and the tube seedlings are easy to survive after transplanting. And the colonization of plantlets grow vigorously and roots doubled, the average flowering period postpones 7 days, and Pantlet withered period postpones about 10 days at late fall or early winter.