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| 马铃薯试管苗及微型种薯形成的几个因素研究 | |
| 引用本文： | 毛碧增,何伯伟,苏善标,李德葆. 马铃薯试管苗及微型种薯形成的几个因素研究[J]. 浙江大学学报(农业与生命科学版), 2002, 28(4): 417-420 |
| 作者姓名： | 毛碧增 何伯伟 苏善标 李德葆 |
| 作者单位： | 1. 浙江大学,生物技术研究所,浙江,杭州,310029 2. 浙江省农业厅,农作物管理局,浙江,杭州,310020 3. 浙江余姚梁辉镇农技站, 浙江,余姚,315400 |
| 基金项目： | 浙江省教育厅资助项目 (2 0 0 10 5 86 )，浙江省种子种苗专项基金资助项目 . |
| 摘 要： | 以马铃薯东农303(Solanum tuberosum L. Dongnong 303)的茎尖为外植体,研究了灭菌方式对茎尖成活率、BA与2,4-D不同浓度组合对诱导不定芽、光强对微型种薯形成以及催芽方式对发芽率的影响. 结果表明, 70%酒精 30s, 0.1% HgCl2 1 min组合茎尖成活率最高; 2 mg/L 2,4-D和0.4 mg/L BA组合最利于诱导不定芽; 弱光环境有利于试管薯的形成; 试管薯经针刺后浸泡于0.5 mg/L 2,4-D和2 mg/L赤霉素混合液8 h的处理,发芽率最高. |
| 关 键 词： | 马铃薯 不定芽 试管薯 |
| 文章编号： | 1008-9209(2002)04-0417-04 |
Study on factors involved in formation of in vitro potato seedlings and microtuber |
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| MAO Bi zeng ,HE Bo wei ,SU Shan biao ,LI De bao. Study on factors involved in formation of in vitro potato seedlings and microtuber[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2002, 28(4): 417-420 | |
| Authors: | MAO Bi zeng HE Bo wei SU Shan biao LI De bao |
| Affiliation: | MAO Bi zeng 1,HE Bo wei 2,SU Shan biao 3,LI De bao 1 |
| Abstract: | The effects of sterilization methods and various combinations of BA and 2,4 D in different concentrations on induction of adventitious buds, of light condition on microtuber formation, and of method of forcing on rate of bud sprouting in potato were studied. The results indicated that the sterilization using 70% ethanol 30 sec, 0.1% HgCl 2 1 min improved survival rate of the stem meristem; the combination of 0.4 mg/L BA and 2 mg/L 2,4 D enhanced adventitious bud induction; low supplemental light increased microtuber yield; thorned microtuber that experienced soaking in a solution of 0.5 mg/L 2,4 D and GA 3 for 8 hours gave the best rate of bud sprouting. |
| Keywords: | Solanum tuberosum L. Dongnong 303 adventitious bud potato microtuber |
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