| 番茄黑环病毒检测方法的建立 |
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| 引用本文： | 高焕利,杨翠云,周国梁,于翠,乔艳艳,马青. 番茄黑环病毒检测方法的建立[J]. 植物检疫, 2007, 21(3): 149-152 |
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| 作者姓名： | 高焕利 杨翠云 周国梁 于翠 乔艳艳 马青 |
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| 作者单位： | [1]西北农林科技大学植保学院陕西省农业分子生物学重点实验室,杨陵,712100 [2]上海出入境检验检疫局,杨陵,712100 |
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| 基金项目： | 上海市科委科技计划;上海出入境检验检疫局项目;教育部长江学者和创新团队发展计划 |
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| 摘 要： | 通过汁液摩擦接种的方法接种几种鉴别寄主,筛选出检测番茄黑环病毒（TBRV）的一套鉴别寄主.同时建立了免疫学电镜检测方法.根据TBRV外壳蛋白基因的保守序列设计2对引物,应用RT-PCR均能从TBRV的6个分离物中扩增到与预期大小相同的DNA片段.利用抽提的质粒研究了RT-PCR方法检测TBRV的灵敏度.
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| 关 键 词： | 番茄黑环病毒(TBRV) 鉴别寄主反应 免疫电镜 灵敏度 |
| 修稿时间： | 2007-01-24 |
The research of detection methods for Tomato black ring nepovirus |
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| Gao Huanli,Yang Cuiyun,Zhou Guoliang,Yu Cui,Qiao Yanyan,Ma Qing. The research of detection methods for Tomato black ring nepovirus[J]. Plant Quarantine, 2007, 21(3): 149-152 |
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| Authors: | Gao Huanli Yang Cuiyun Zhou Guoliang Yu Cui Qiao Yanyan Ma Qing |
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| Abstract: | The diagnostic species were selected using virous partile to inculate the indicator plants.Electron microscopy observation was established.RT-PCR amplification was established using two primers pair specific to the conserved sequence of TBRV coat protein gene produced a specific band with a expected size from the 6 isolates of TBRV.Sequence determination and analysis was showed that the determined sequence is partial TBRV coat protein gene.The Sensitivity of TBRV is determined using dilution of Plasmind of TBRV by RT-PCR. |
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| Keywords: | RT-PCR |
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