| 饲料中三聚氰胺的ELISA检测方法的探讨 |
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| 引用本文： | 郭洁,李宏,贾青,杨汉卿,陈瑛,冯西辉. 饲料中三聚氰胺的ELISA检测方法的探讨[J]. 中国农业科技导报, 2009, 11(Z1): 50-51 |
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| 作者姓名： | 郭洁 李宏 贾青 杨汉卿 陈瑛 冯西辉 |
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| 作者单位： | (1.农业部饲料质量监督检验测试中心, 西安 ,710016,2.铜川市印台区畜牧站, 陕西 铜川 727000) |
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| 摘 要： | 试验利用BRAXIS试剂盒和自制（经气相色谱质谱法确证不含三聚氰胺）三聚氰胺添加量为2 mg/kg的配合料和浓缩料作为阳性对照样品,对100批次饲料产品的进行了三聚氰胺筛选,并将两种方法的筛选结果按照NY/T1372-2007中的气相色谱质谱法进行了确证和比较。试验结果表明：利用三聚氰胺添加量为2 mg/kg配合料和浓缩料为阳性对照样品,对样品的筛选结果,要比使用BRAXIS试剂盒的内制曲线筛选的结果,产生的假阳性少,准确率相对要高。
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| 关 键 词： | 三聚氰胺 检测 ELISA方法, |
| 收稿时间： | 2009-10-15 |
| 修稿时间： | 2009-11-20 |
Discussion on Melamine Determination in Feed by ELISA Method |
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| GUO Jie,LI Hong,JIA Qing,YANG Han-qing,CHEN Ying,FENG Xi-hui. Discussion on Melamine Determination in Feed by ELISA Method[J]. Journal of Agricultural Science and Technology, 2009, 11(Z1): 50-51 |
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| Authors: | GUO Jie LI Hong JIA Qing YANG Han-qing CHEN Ying FENG Xi-hui |
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| Affiliation: | (1.Feed Quality Supervision and Testing Center, Ministry of Agriculture, Xian 710016|2.Tongchuan City Yintai District Animal Husbandry Station, Shaanxi Tongchuan 727000, China) |
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| Abstract: | The research made use of BRAXIS kit and self-made compound and concentrated feed(via gas chromatography-mass spectrometry confirmed melamine-free) that melamine adding amount is 2 mg/kg as a positive control sample, screened melamine in 100 batches of pig and chicken feed products. Screening results of two methods in accordance were confirmed and compared with NY/T1372-2007 gas chromatography-mass spectrometry. The results showed that: the use of compound and concentrated feed that melamine adding amount is 2mg/kg as a positive control sample, the results were in fewer false-positive rate and higher accuracy than the use of BRAXIS Kit for the results of screening within the system of curves. |
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| Keywords: | melamine determination ELISA method |
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