| 毛白杨离体快速繁殖简报 |
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| 引用本文： | 孙永明,张宝国.毛白杨离体快速繁殖简报[J].山西林业科技,1999(2):46-48. |
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| 作者姓名： | 孙永明 张宝国 |
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| 作者单位： | 山西省林业科学研究院!山西太原030012(孙永明，苏淑云，刘劲，王文英)，太原市林场!山西太原030009(张宝国) |
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| 摘 要： | 以河北省易县毛白杨为试材，对其离体快速繁殖过程中的各阶段进行了较为系统的观察分析。结果表明： 幼树半木质化新梢中部单芽茎段为最佳外植体材料； 启动培养基以改良培养基 Ｂ５＋ ０２ ｍ ｇ／ Ｌ Ｎ Ａ Ａ为最好； 试管苗的叶片可与腋芽及茎尖同时作为继代培养的材料， 最佳分化培养基为 Ｍ Ｓ＋ ０２５ ｍ ｇ／ Ｌ Ｂ Ａ＋ ０２５ ｍ ｇ／ Ｌ Ｚｔ＋ ０２５ ｍ ｇ／ Ｌ Ｉ Ａ Ａ；最佳生根培养基为１／２ Ｍ Ｓ＋ ００２ ｍ ｇ／ Ｌ Ｎ Ａ Ａ＋ １０ ｍ ｇ／ Ｌ Ｖ Ｂ１＋１５％ 蔗糖， 生根率可达１００％ ， 生根数量为４ 条。
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| 关 键 词： | 毛白杨 繁殖 组织培育法 |
Report on in Vitro Quick propagation of Populus tomentosa |
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| SUN Yong ming,ZHANG Bao guo,SU Shu yun,LIU jin,WANG Wen ying.Report on in Vitro Quick propagation of Populus tomentosa[J].Shanxi Forestry Science and Technology,1999(2):46-48. |
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| Authors: | SUN Yong ming ZHANG Bao guo SU Shu yun LIU jin WANG Wen ying |
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| Institution: | SUN Yong ming 1 ZHANG Bao guo 2 SU Shu yun 1 LIU jin 1 WANG Wen ying 1 |
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| Abstract: | Observed and analyzed the in vitro quick propagation process of Populus tomentosa from Yixian of Hebei province.The results showed that:the best explant material is the single bud stem section from the middle of sapling's semi lignified new growth,modified B 5 0 2mg/L NAA is the best initialization medium.Tube seedling's leaf blade,axillary bud,and stem apex can be used as subculture materials at the same time.The best differentiation medium is MS 0 25mg/L BA 0 25mg/L Zt 0 25mg/L IAA,and best rotting medium is 1/2 MS 0 02mg/L NAA 10mg/L VB 1 1 5% sucrose,rooting rate up to 100% and maximum rooting number 4. |
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| Keywords: | Populus tomentosa propagation tissue culture |
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