GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum |
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| Authors: | Hun-Su Seo Kyoung Hee Kim Dae-Yong Kim Bong-Kyun Park Nam-Shik Shin Jae-Hoon Kim Heejeong Youn |
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| Affiliation: | 1.Pfizer Animal Health, Seoul 135-784, Korea.;2.College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.;3.College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. |
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| Abstract: | We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica. |
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| Keywords: | GC/MS HPLC Neospora caninum Sophora flavescens Torilis japonica |
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