| 鸡致病性MDV感染AGP法检测可靠性研究 |
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| 引用本文： | 张训海,朱鸿飞,卢春,陈溥言,蔡宝祥.鸡致病性MDV感染AGP法检测可靠性研究[J].中国农业科学,1999,19(4):107-109. |
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| 作者姓名： | 张训海 朱鸿飞 卢春 陈溥言 蔡宝祥 |
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| 作者单位： | 1. 安徽农业技术师范学院动物科学系,凤阳,233100
2. 南京农业大学动物医学院 |
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| 基金项目： | 国家自然科学基金,安徽省自然科学基金,安徽省教委资助 |
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| 摘 要： | 常规琼脂扩散（ＡＧＰ）试验通过羽毛囊马立克氏病病毒（ＭＤＶ）特异抗原的检出来诊断鸡致病性ＭＤＶ的感染〔１，２，６〕，因特异性强、操作简便而广泛应用。但其检出率与感染鸡群在不同时期的检测〔３〕以及火鸡疱疹病毒（ＨＶＴ）疫苗免疫状态〔４〕密切相关。此现象...
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| 关 键 词： | 马立克氏病病毒抗体 羽毛囊 琼脂扩散试验 |
| 修稿时间： | 1998-01-05 |
Study on Reliability of the Detection of AGP for Virulent MDV-infection of Chickens |
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| Zhang Xunhai,Zhu Hongfei,Lu Chun,Chen Puyan,Cai Baoxiang.Study on Reliability of the Detection of AGP for Virulent MDV-infection of Chickens[J].Scientia Agricultura Sinica,1999,19(4):107-109. |
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| Authors: | Zhang Xunhai Zhu Hongfei Lu Chun Chen Puyan Cai Baoxiang |
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| Abstract: | 5 chickens of 1-day-old vaccinated with HTV and 10 1-day-old unvaccinated chickens were inoculated with Marek's disease virus (MDV) strain Beijing-1(BJ-1) at 15 days of age,respectively.During 14～70 days post-inoculation(PI), MDV antigen and/or antibody in the feather follicle and blood were monitored by agar-gel precipitation (AGP) assay.The results showed that the antibody was identified in the feather-pulp of chickens challenged at 49 days PI and vaccinated challenged at 30 days PI.Growth and decline of the antibody were positively related with that of blood while the activity of the antigens was negatively related with that of the antibody in the feather follicle. However, MDV DNA were always identified by dot-blot hybridization in the feather tips with activity of the antibody. |
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| Keywords: | MDV antibody Feather follicle Agar gel precipitation (AGP) |
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